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Method for dissolving mid-low-grade rock phosphate by utilizing mixed bacterial liquid

A technology of mixing bacterial liquid and phosphate rock powder, applied in the direction of bacteria, etc., can solve the problems of consuming large amounts of organic nutrients, slow dissolution of phosphate rock powder, and weak organic acidity, and achieves low price, environmental friendliness, and strong Asia Effect of Iron or Sulfur Oxidation Ability

Inactive Publication Date: 2013-06-05
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth of heterotrophic bacteria needs to consume a large amount of organic nutrients, and the organic acid produced by metabolism is not strong, the speed of dissolving phosphate rock powder is slow, and the phosphorus dissolution rate is low. These deficiencies make it unnecessary to consume a large amount of organic nutrients and Inorganic chemoautotrophic microorganisms (autotrophs) that can produce more acidic inorganic acids have attracted more and more attention, such as Thiobacillus microorganisms, etc.
However, the research on the use of autotrophic bacteria to dissolve medium and low-grade phosphate rocks is still in its infancy, and the strains used are limited to Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, etc. Mesophilic bacteria, phosphorus dissolution rate is still low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:

[0032] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 15% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, and the pH of the sulfuric acid regulation substratum A with concentration is 20wt% is 2.2, add the original bacterial liquid of Sulfobacillus thermophilic sulfur oxidans; then place the container in a constant temperature shaker at 45°C, shake and culture at 140 rpm for 5 days, and obtain the activated bacteri...

Embodiment 2

[0048] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:

[0049] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 18% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, be that the pH of the sulfuric acid regulation substratum A with concentration is 10wt% is 2.8, then add the original bacterial liquid of Sulfobacillus thermophilic sulfur oxidans; then place the container in a constant temperature shaker at 50°C, shake and culture at 160 rpm for 4 days to obtain activated bact...

Embodiment 3

[0062] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:

[0063] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 20% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, and the pH of the sulfuric acid regulation substratum A with concentration is 15wt% is 3.0, then add the original bacterial liquid of Sulfobacillus thermophilic sulfur-oxidizing bacteria; then place the container in a constant temperature shaker at 53°C, and vibrate at 140 rpm for 3 days to obtain activated bac...

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Abstract

The invention relates to a method for dissolving mid-low-grade rock phosphate by utilizing mixed bacterial liquid. The method is characterized by comprising the following steps: 1) activating original sulfobacillus thermosulfidooxidans liquid to obtain activated sulfobacillus thermosulfidooxidans liquid; 2) activating original leptospirillum ferriphilum liquid to obtain activated leptospirillum ferriphilum liquid; 3) activating original acidithiobacillus caldus liquid to obtain activated acidithiobacillus caldus liquid; 4) mixing and domesticating the activated sulfobacillus thermosulfidooxidans liquid, the activated leptospirillum ferriphilum liquid and the activated acidithiobacillus caldus liquid to obtain the domesticated mixed bacterial liquid; and 5) dissolving the mid-low-grade rock phosphate in the domesticated mixed bacterial liquid to obtain the soluble phosphorus. The method has the characteristics of high phosphorus solubility, simple process and low production cost.

Description

technical field [0001] The invention relates to a method for dissolving medium and low-grade phosphate rock powder by using mixed bacterial solution. Background technique [0002] Phosphorus is one of the essential nutrients for crop growth and an important material guarantee for agricultural production. However, according to statistics, 43% of the world's agricultural soil is deficient in phosphorus, and the area of ​​phosphorus deficient agricultural soil in my country is as high as 79%. Soil phosphorus deficiency severely limits the yield and quality of crops. Therefore, in order to promote agricultural production, a large amount of phosphorus fertilizer must be invested. [0003] Phosphorus exists in large quantities in phosphate rocks, and its main mineral forms are hydroxyapatite, fluoroapatite, and carbonate apatite, etc., which constitute the main components of phosphate rock. Phosphate rocks that exist in large quantities in nature provide cheap raw materials for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 池汝安肖春桥方玉娟
Owner WUHAN INSTITUTE OF TECHNOLOGY
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