Method for dissolving mid-low-grade rock phosphate by utilizing mixed bacterial liquid
A technology of mixing bacterial liquid and phosphate rock powder, applied in the direction of bacteria, etc., can solve the problems of consuming large amounts of organic nutrients, slow dissolution of phosphate rock powder, and weak organic acidity, and achieves low price, environmental friendliness, and strong Asia Effect of Iron or Sulfur Oxidation Ability
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Embodiment 1
[0031] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:
[0032] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 15% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, and the pH of the sulfuric acid regulation substratum A with concentration is 20wt% is 2.2, add the original bacterial liquid of Sulfobacillus thermophilic sulfur oxidans; then place the container in a constant temperature shaker at 45°C, shake and culture at 140 rpm for 5 days, and obtain the activated bacteri...
Embodiment 2
[0048] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:
[0049] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 18% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, be that the pH of the sulfuric acid regulation substratum A with concentration is 10wt% is 2.8, then add the original bacterial liquid of Sulfobacillus thermophilic sulfur oxidans; then place the container in a constant temperature shaker at 50°C, shake and culture at 160 rpm for 4 days to obtain activated bact...
Embodiment 3
[0062] A method for dissolving medium and low-grade phosphate rock powder by using a mixed bacterial solution, comprising the steps of:
[0063] 1) Activation of the original bacterial liquid of Sulfobacillus thermosulfidooxidans: The amount of the original bacterial liquid of Sulfobacillus thermosulfidooxidans is medium A (the composition of medium A is: 3.0g (NH 4 ) 2 SO 4 , 1.0gMgSO 4 .7H 2 O, 0.2g K 2 HPO 4 , 0.2g KCl, 0.02g Ca(NO 3 ) 2 , 0.2g yeast extract, 30g FeSO 4 .7H 2 (2 and 1000mL sterile water) 20% of the volume, choose the original bacterium liquid and substratum A of sulfobacillus thermophilic thiooxidans; substratum A is packed in the container, and the pH of the sulfuric acid regulation substratum A with concentration is 15wt% is 3.0, then add the original bacterial liquid of Sulfobacillus thermophilic sulfur-oxidizing bacteria; then place the container in a constant temperature shaker at 53°C, and vibrate at 140 rpm for 3 days to obtain activated bac...
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