Method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes

Abstract

The invention belongs to the field of molecular biology, and discloses a method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes. In prior arts, a technology of hydrolyzing pig haemproteins with single enzyme reaches a hydrolysis degree of only 10%, while deer blood can be directly used only. The method of the present invention comprises the following steps: (1) using original deer blood or deer blood powder as a raw material, preparing a deer blood solution according to a concentration of 5% to 10% of the deer blood component mass; (2) adding papain to the prepared deer blood solution for hydrolyzing under a hydrolysis temperature of 35 to 40 DEG C, a pH value of 6.5 to 7.5, and a hydrolysis time of 2 to 3 hours, wherein the added amount equals 2% to 6% of the deer blood component mass, such that a first hydrolysate is obtained; (3) adding alkali protease to the first hydrolysate for hydrolyzing under a hydrolysis temperature of 50 to 55 DEG C, a pH value of 8.5 to 9.5, and a hydrolysis time of 2 to 3 hours, wherein the added amount equals 2% to 6% of the deer blood component mass, such that a second hydrolysate is obtained; (4) deactivating the residual enzyme through a water-bath, purifying the obtained solution with a membrane technology, such that a bioactive peptide solution is obtained.

Description

technical field [0001] The invention relates to a method for sequentially hydrolyzing deer blood proteins by two enzymes to obtain active peptides, which realizes the biological utilization of deer blood resources and obtains peptide products with high biological activity, and belongs to the field of molecular biology. Background technique [0002] Compounds formed by linking amino acids with each other through amide bonds are called peptides. Peptides containing more than 10 amino acid residues are called polypeptides, while those containing less than 10 amino acid residues are called oligopeptides or oligopeptides. Because peptide molecules are much smaller than proteins, but have high biological activity, they are also called biologically active peptides. Bioactive peptides have certain physiological functions on the body, and play the role of transmitting physiological information and regulating physiological functions in life activities, and are of great significance to...