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Simple fermentation method for acarbose

A fermentation method, acarbose technology, applied in the field of acarbose fermentation, can solve the problems such as no literature reports

Inactive Publication Date: 2013-07-24
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no literature reporting on the control strategy of acarbose fermentation on an industrial scale.

Method used

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  • Simple fermentation method for acarbose
  • Simple fermentation method for acarbose
  • Simple fermentation method for acarbose

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Embodiment Construction

[0025] The synthetic pathway of acarbose shows that maltose and glucose are the precursors of acarbose, and they are directly combined into the molecular structure of acarbose. Therefore, the ratio of maltose and glucose plays an important role in the synthesis of acarbose. In order to explore the ratio of maltose and glucose to actinomycetes ( Actinoplanessp ) to produce acarbose by fermentation, using maltose and glucose as the carbon source of the feed medium, using seven different ratios, the mass ratios are 1:1, 1:0, 2:1, 3: 1, 4:1, 5:1 and 0:1. During the fed-batch fermentation in shake flasks, each shake flask was supplemented with the above ratio of feed medium at 72, 96 and 120 h, so that the total sugar concentration reached 30 g / L each time, The specific experimental results and variance analysis results are shown in Table 1.

[0026] Table 1 Effect of the ratio of maltose and glucose in the feed medium on the fermentation of acarbose

[0027]

[0028] a :...

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Abstract

A simple fermentation method for acarbose, using Actinoplanessp or bacteria of the same genus as the fermentation bacteria, and the fermentation steps include: (1) shaking flask fermentation, (2) 100-L tank fermentation, (3) fermentation on 30-m3 tanks. The simple and easy fermentation method of acarbose described in the present invention, first, adopts Actinoplanessp or other strains of the same genus to study its fed-batch fermentation to produce acarbose on shake flasks and 100-L fermenters The carbon source control strategy of wave sugar, the results showed that the total sugar control concentration in the feeding stage, especially the concentration of maltose and glucose had a significant impact on the synthesis of acarbose. Based on this, the two key parameters of total sugar and reducing sugar (maltose and glucose) were screened out as scale-up factors, and the scale-up of industrial fermentation of acarbose from 100-L tanks to 30-m3 tanks was successfully realized.

Description

technical field [0001] The invention relates to a fermentation method of acarbose, specifically utilizing actinomycetes ( Actinoplanessp ) studied for its 30-m 3 An industrial scale-up method for producing acarbose by fermentation on a tank. Background technique [0002] Acarbose is produced by Actinomycetes mobilis ( Actinoplanessp ) The pseudotetrasaccharide substance produced by fermentation can compete with α-glucosidase (Wehmeier UF, Piepersberg W. Biotechnology and molecular biology of the α-glucosidase inhibitor acarbose[J]. Appl Microbiol Biotechnol, 2004, 63: 613–625). Because acarbose is difficult to digest in the human body, and it has not been found to be toxic to the human body, acarbose is widely used in the treatment of type 2 diabetes to achieve the purpose of reducing postprandial hyperglycemia in diabetic patients (Hanefeld M, Schaper F, Koehler C. Effect of Acarbose on Vascular Disease in Patients with Abnormal Glucose[J]. Tolerance Cardiovasc Drugs Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/045
Inventor 李昆太程新
Owner JIANGXI AGRICULTURAL UNIVERSITY
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