Simple fermentation method for acarbose

A fermentation method and acarbose technology, applied in the field of acarbose fermentation, can solve problems such as no literature reports

Inactive Publication Date: 2011-10-19
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no literature reporting on the control strategy of acarbose fermentation on an industrial scale.

Method used

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  • Simple fermentation method for acarbose
  • Simple fermentation method for acarbose
  • Simple fermentation method for acarbose

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Embodiment Construction

[0025] The synthetic pathway of acarbose shows that maltose and glucose are the precursors of acarbose, and they are directly combined into the molecular structure of acarbose. Therefore, the ratio of maltose and glucose plays an important role in the synthesis of acarbose. In order to explore the ratio of maltose and glucose to actinomycetes ( Actinoplanessp ) to produce acarbose by fermentation, using maltose and glucose as the carbon source of the feed medium, using seven different ratios, the mass ratios are 1:1, 1:0, 2:1, 3: 1, 4:1, 5:1 and 0:1. During the fed-batch fermentation in shake flasks, each shake flask was supplemented with the above ratio of feed medium at 72, 96 and 120 h, so that the total sugar concentration reached 30 g / L each time, The specific experimental results and variance analysis results are shown in Table 1.

[0026] Table 1 Effect of the ratio of maltose and glucose in the feed medium on the fermentation of acarbose

[0027]

[0028] a: The...

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Abstract

The invention discloses a simple fermentation method for acarbose. The method adopts actinoplanessp or a strain of the same species of the actinoplanessp as a fermentation strain. The method comprises fermentation steps of: (1) shaking flask fermentation, (2) carrying out fermenting in a 100-L fermenter, (3) carrying out fermenting in a 30-m<3> fermenter. According to the simple fermentation method for the acarbose provided by the present invention, a carbon source control strategy for fed-batch fermentation of the acarbose is researched through adopting the actinoplanessp or other strains ofthe same species of the actinoplanessp, and research results show that a controlled concentration of total sugar in a feeding phase, especially the controlled concentrations of maltose and glucose have remarkable effects on the synthesis of the acarbose. Based on this point, two key parameters of the total sugar and reducing sugar (the maltose and the glucose) are screened as amplification factors, such that an industrial fermentation scale-up of the acarbose from the 100-L fermenter to the 30-m<3> fermenter is realized.

Description

technical field [0001] The invention relates to a fermentation method of acarbose, specifically utilizing actinomycetes ( Actinoplanessp ) studied for its 30-m 3 An industrial scale-up method for producing acarbose by fermentation on a tank. Background technique [0002] Acarbose is produced by Actinomycetes mobilis ( Actinoplanessp ) The pseudotetrasaccharide substance produced by fermentation can compete with α-glucosidase (Wehmeier UF, Piepersberg W. Biotechnology and molecular biology of the α-glucosidase inhibitor acarbose[J]. Appl Microbiol Biotechnol, 2004, 63: 613–625). Because acarbose is difficult to digest in the human body, and it has not been found to be toxic to the human body, acarbose is widely used in the treatment of type 2 diabetes to achieve the purpose of reducing postprandial hyperglycemia in diabetic patients (Hanefeld M, Schaper F, Koehler C. Effect of Acarbose on Vascular Disease in Patients with Abnormal Glucose[J]. Tolerance Cardiovasc Drugs Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12R1/045
Inventor 李昆太程新
Owner JIANGXI AGRICULTURAL UNIVERSITY
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