Method for fermenting high temperature-resistant phytase genetic engineering bacteria

A technology of genetically engineered bacteria and fermentation method, which is applied in the field of fermentation of high temperature-resistant phytase genetically engineered bacteria, can solve the problems of high price and late phytase, and achieve the effects of high recovery rate, simple steps and stable process

Inactive Publication Date: 2011-03-16
广州市微生物研究所
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Phytase is found in animals, plants and microorganisms, but the real development value is limited to the phytase produced by microbial fermentation
Domestic research on phytase started late, and most of the high-quality phytase preparations used in the market are imported products and are expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] (1) High-density culture stage of heat-resistant phytase genetically engineered bacteria

[0024] Inoculate the seeds of phytase genetically engineered bacteria in a 5000L automatic fermenter with 10% inoculum amount, cultivate at 30°C, adjust the pH to 5.5 with 14% ammonia water, adjust the speed and ventilation to control the dissolved oxygen to be greater than 20%; After 16 hours of fermentation and cultivation, the dissolved oxygen rose rapidly. At this time, the carbon source glucose was added in a constant dissolved oxygen manner, and the feeding was completed after 32 hours of cultivation.

[0025] (2) Methanol-induced expression of thermostable phytase

[0026] After the high-density cultivation of phytase genetically engineered bacteria is over, the carbon source glucose is exhausted, and the dissolved oxygen rises, then 8 mL / L / h of methanol is added to induce the expression of phytase, and the pH is adjusted to pH 6.0 with concentrated ammonia water. Adjust ...

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PUM

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Abstract

The invention discloses a method for fermenting high temperature-resistant phytase genetic engineering bacteria. High-density culturing of the high temperature-resistant phytase genetic engineering bacteria is performed by using a full-automatic fermentation tank system, low-cost culture medium components and constantly dissolved oxygen fed-batch technology, so that high-density expression of high temperature-resistant phytase is realized. Compared with the prior art, the method of the invention can efficiently express the high temperature-resistant phytase in a large scale, an obtained phytase product has high high-temperature resistance, residual enzyme activity is over 69 percent after heat is preserved at the temperature of 90 DEG C for 20 minutes due to the heat resistance of the product, and the product is superior to the current commercially available commodity enzyme preparation and can meet the requirement of high temperature feed granulation.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a fermentation method of high-temperature resistant phytase genetically engineered bacteria. Background technique [0002] Monogastric animals lack digestive enzymes that release inorganic phosphorus from the hydrolysis of phytic acid molecules, and plant phosphorus, which accounts for 50-70% of the total phosphorus in animal diets, cannot be fully utilized, and the phosphorus ecological burden on land and water resources is becoming increasingly serious due to discharge into nature; Salts can reduce the utilization rate of main trace elements such as zinc, manganese, iron, calcium and potassium by animals through chelation; by combining with proteins to form phytic acid complexes, the digestion and absorption rate of proteins by animals can be reduced, thereby reducing feed utilization Rate. Phytase is a general term for a class of enzymes that catalyze the hydrolysis of phytic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16
Inventor 黄魁英明飞平夏枫耿梁淑娃彭中健黄乐天蓝碧锋缪承杜赵培静
Owner 广州市微生物研究所
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