Method for measuring total cell wall matter content of tobacco

Abstract

The invention discloses a method for measuring the total cell wall matter content of tobacco, which belongs to the technical field of tobacco analysis and detection. The method comprises the following steps of: weighing a certain number of crushed tobacco samples with the fineness of 40 to 80 meshes, wrapping the crushed tobacco samples by using 1,000-mesh nylon filter cloth and tightening the wrapped samples; arranging 24 tobacco samples into a Soxhlet extraction device; adding 2.0 liters of mixed solution of petroleum ether and acetone, performing Soxhlet extraction for 48 hours, and observing extract liquor by using eyes until the extract liquor is colorless; arranging the Soxhlet-extracted samples into a beaker into which water in an amount 20 times the weight of the samples is added, heating the solution until the solution is boiling, keeping boiling the solution for 1 hour, performing desugaring, filtering the solution by using an abrased glass crucible, discarding the water and squeezing the samples to dryness by using hands; repeating the operations for four or five times; arranging the extracted samples into a drying oven, and drying the samples for 2.5 hours at 100 DEG C; and arranging the dried samples into a dryer with allochroic silicagel, cooling the samples to room temperature, weighing the samples, and obtaining the total weight of cell wall matters of the tobacco by subtracting the weight of the wrapping nylon filter cloth from the weight obtained by the weighing. The method has the advantages of simplicity, fewer toxic hazards and pollutions, low cost, high efficiency and analysis data accuracy.

Description

Technical field: [0001] The invention relates to a method for measuring the content of total cell wall substances in tobacco, belonging to the technical field of tobacco detection. Background technique: [0002] Tobacco leaf total cell wall material is a kind of inactive, structurally stable insoluble material, which provides a structural framework for tobacco plants during the growth and development of tobacco. As far as flue-cured tobacco leaves are concerned, the content of cell wall material accounts for about 1 / 3 of the dry matter, and accounts for ±43% in tobacco stems. Tobacco cell wall substances mainly include cellulose, hemicellulose, pectin and lignin. These substances have varying degrees of influence on the physical properties of tobacco leaves, indirectly determine the processing characteristics of tobacco leaves, and also affect the smoking quality of tobacco. When the total cell wall substance content is appropriate, it is beneficial to the combustion of to...

AI Technical Summary

Problems solved by technology

After the above method of degreasing, the residue needs to be heated and refluxed with water to remove the sugar. After each elution, it needs to be filtered with a glass sand crucible, and then replaced with new water and repeated treatment. It takes 4-5 times to remove the sugar. The operation is troublesome and cumbersome Secondary transfers can result in sample loss