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Antrodia camphorata cyclohexenone compound for suppressing growth of bladder cancer tumor cells

A technology of tumor cells and compounds, applied in the new application field of compounds, can solve unpublished specific problems and so on

Active Publication Date: 2011-11-09
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its constituents have been analyzed one after another, no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomea. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cintrodia Cintrodia anticancer, and to apply Antrodia Cinnamomea to cancer such as bladder. Cancer treatment and prevention are of great help

Method used

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  • Antrodia camphorata cyclohexenone compound for suppressing growth of bladder cancer tumor cells
  • Antrodia camphorata cyclohexenone compound for suppressing growth of bladder cancer tumor cells
  • Antrodia camphorata cyclohexenone compound for suppressing growth of bladder cancer tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0023] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.

[0024] The Antrodia camphorata extract collected above was analyzed using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP 18 column, and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) ) as the mobile phase (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio 10% to 90%; 35 to 40 minutes, the B ratio ...

Embodiment 2

[0027] Anti-bladder cancer tumor cell activity test in vitro

[0028] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium containing human bladder cancer tumor cell TSGH-8301 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the known MTT analysis method, and the bladder cancer tumor cell TSGH-8301 is a human bladder cancer cell line (human bladder carcinoma cell line).

[0029] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow ...

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Abstract

The invention relates to a new use of a compound. In the invention, (4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone is obtained from the extract of Antrodia camphorate by separation and purification, and the cyclohexenone compound can be used for suppressing the growth of the bladder cancer tumor cells and can be used in medical compositions for suppressing the growth of bladder cancer tumor cells.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of the compound isolated and purified from the extract of Antrodiacinnamomea to inhibit the growth of bladder cancer tumor cells. Background technique [0002] According to epidemiological surveys, bladder cancer is the most common cancer among genitourinary system tumors, and its incidence is increasing year by year. It often occurs in men, and the incidence of men is usually two to five times that of women. times. The occurrence of bladder cancer is related to many factors, and according to statistics, it is closely related to frequent exposure to chemical carcinogens such as aniline (aniline) or aromatic amine (aromatic amine) compounds in dyes, such as painters, Leather workers, truck drivers, and plumbers are more susceptible to bladder cancer. Other risk factors include smoking, coffee, tea, painkillers, artificial sweeteners, or chronic urinary tract inflam...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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