Kit for detecting Southeast Asia type alpha-thalassemia

A technology of thalassemia and detection kits, applied in biological testing, material inspection products, etc., can solve the problems of Pap edema fetus, heavy burden on family and society, etc.

Active Publication Date: 2011-11-23
ZHUHAI S E Z HAITAI BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most of these carriers have no obvious symptoms, if both men and women are carriers, their children may have t

Method used

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  • Kit for detecting Southeast Asia type alpha-thalassemia
  • Kit for detecting Southeast Asia type alpha-thalassemia
  • Kit for detecting Southeast Asia type alpha-thalassemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] specific implementation plan

[0012] [Main components of the kit]

[0013]

[0014] 1. Preparation method of sample treatment solution: Weigh 8.3g ammonium chloride and 2.0g casein respectively, add 40ml glycerin, then add purified water to 1L, stir until fully dissolved.

[0015] 2. How to use the kit

[0016] Use sterile anticoagulated venous blood. For samples from patients with severe anemia, the ratio of plasma to red blood cells in the sample should be adjusted to 1:1, and then diluted as required.

[0017] 2.1 Kit reaction reagent preparation

[0018] 2.1.1 Microwell strips

[0019] Fix the number of microwell strips required for detection on the plate holder, and store the remaining microwell strips in sealed bags.

[0020] 2.1.2 Washing solution

[0021] Dilute the concentrated wash solution in a clean graduated cylinder. Add distilled or deionized water to 50ml of concentrated washing liquid to make up to 500ml, and mix well.

[0022] 2.1.3 Other ...

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Abstract

The invention relates to a kit for detecting Southeast Asia type alpha-thalassemia and discloses a sample treating fluid of the kit. The kit is characterized in that in the sample treating fluid, the content W/V of ammonium chloride is 0.83%, the required content W/V of casein ranges from 0.2% to 0.8% and the content V/V of glycerin ranges from 4% to 6%. The kit can conveniently and effectively detect Zeta globin chains and assist in diagnosing the Southeast Asia type alpha-thalassemia.

Description

technical field [0001] The invention relates to a detection kit for detecting Southeast Asian type α-thalassemia and its application. Background technique [0002] Thalassemia is divided into alpha type and beta type. The Southeast Asian gene carriers of the α type are most widely distributed in Southeast Asia and southern my country, and are the main type causing potential harm. Although most of these carriers have no obvious symptoms, if both men and women are carriers, their children may have the possibility of Papanicolaou edema and hemoglobin H disease, which will bring a heavy burden to the family and society. [0003] It is known that the hemoglobin Zeta chain exists in the fetal period of normal people, and it cannot be detected 3 months after birth, but the Zeta chain exists in the blood of Southeast Asian α-thalassemia gene carriers for life. Therefore, Zeta chain can be used as a marker protein for auxiliary diagnosis of α-thalassemia in Southeast Asia. [0004...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/68
Inventor 陈爽汤汉文
Owner ZHUHAI S E Z HAITAI BIOLOGICAL PHARMA
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