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A method of making a burn animal model

An animal model and preparation method technology, applied in the field of medical and life science experiments and research, can solve the problems of difficult control of burn degree, lack of exact dosage and burn time, and difficulty in purchasing

Inactive Publication Date: 2011-12-14
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently commonly used burn model fuels are difficult to purchase, and most of them have no exact dosage and burn time, and the burn degree is difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: 20% superficial second-degree burns in rats caused by mixed fuel

[0012] Preparation of mixed fuel: Accurately weigh 35g of petroleum refining residue, that is, atmospheric residue, and add 93 # Add gasoline to 100g, put it in a grinding bottle, and stir thoroughly. Put it in the refrigerator, fully shake it for 10 minutes every day, for a total of 7 days, and it becomes a uniform gel.

[0013] Experimental animals: SD rats, male or female, weighing 200-250 g, provided by the Experimental Animal Research Center of Fourth Military Medical University.

[0014] Skin treatment: (1) Hair removal: 3% pentobarbital sodium 30mg / kg ip anesthesia, the rat was fixed on the board, and the hair was removed with an electric tweezer, slightly larger than the area to be burned. (2) Hair removal: use sodium sulfide paste to remove hair and set it aside.

[0015] Calculation of body surface area: Accurately weigh the body weight and fast for 16 hours before weighing. Calc...

Embodiment 2

[0021] Embodiment 2: 20% body surface area deep second-degree burns in rats caused by mixed fuel

[0022] Mixed fuel preparation, experimental animals, skin treatment, etc. are the same as in Example 1.

[0023] Model making: Animals were anesthetized with 3% pentobarbital sodium 30 mg / kg ip, and the area around the pre-burned skin was covered with a damp cloth. Use a brush to evenly apply the mixed fuel to the pre-burn area. The paint area is consistent with the pre-burn area. Ignite immediately and count with a stopwatch. The burn time is set to 25 seconds. When the time comes, use wet gauze to extinguish the fire; environment. Immediately after the injury, 5ml lactated Ringer's solution was injected into the intraperitoneal cavity, reared in separate cages, and free to eat and drink.

[0024] Determining the depth of burn is the same as in Example 1.

[0025] Diagnosis results: immediately after the burn, the wound surface turned white, slightly swollen, and the gloss wa...

Embodiment 3

[0026] Embodiment 3: 20% body surface area III degree burns of rats caused by mixed fuel

[0027] Mixed fuel preparation, experimental animals, skin treatment, etc. are the same as in Example 1.

[0028] Model making: Animals were anesthetized with 3% pentobarbital sodium 30 mg / kg ip, and the area around the pre-burned skin was covered with a damp cloth. Use a brush to evenly apply the mixed fuel to the pre-burn area. The paint area is consistent with the pre-burn area. Ignite immediately and count with a stopwatch. The burn time is set to 30 seconds. When the time comes, use wet gauze to extinguish the fire. Combustion needs to be carried out in an environment with stable airflow. Immediately after the injury, 5ml lactated Ringer's solution was injected into the intraperitoneal cavity, reared in separate cages, and free to eat and drink.

[0029] Determining the depth of burn is the same as in Example 1.

[0030] Diagnosis results: The area of ​​the skin after the injury w...

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PUM

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Abstract

The invention relates to a method for producing a burnt animal model with a mixed fuel, which is characterized in that the mixed fuel is composed of 20% to 60% of petroleum refining residue and 40% to 80% of gasoline, and the preparation method is that the petroleum refining residue is dissolved in gasoline , mix well, freeze at -20°C, take it out, stir, freeze and stir repeatedly 3 to 7 times until it becomes a uniform jelly; apply the mixed fuel evenly to rats, mice, guinea pigs, rabbits, pigs, dogs and other animals for hair removal and pre-burning The skin surface was ignited and burned continuously for 15-30 seconds; fluid resuscitation was performed immediately after the injury, cages were kept, and food and drink were free; the pathological sections of the wound were observed under a light microscope and the burn grade was determined; this model is easy to operate and reproducible. With the advantages of high success rate and easy control of burn degree, it can be used for research on the pathological mechanism of human burns, screening and evaluation of therapeutic drugs, etc.

Description

technical field [0001] The invention relates to a method for making a burn animal model by using petroleum refining residues, which is used for the research on the pathological mechanism of human burns, the screening of drugs and the therapeutic effect. The invention belongs to the field of medical and life science experimental research. Background technique [0002] Burns are an extremely complex traumatic disease caused by physical, chemical, radiation, laser and many other factors acting on the body. Despite human efforts to prevent burns, the number of burn patients received by emergency rooms is still large every year. According to statistics, there are as many as 500,000 burn cases in the United States every year. Due to the limitations of accumulated clinical experience and the complexity of the occurrence and development of burns, the research on the pathological mechanism and therapeutic drugs of burns is still one of the key directions of medical research. Due to...

Claims

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Application Information

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IPC IPC(8): A61D1/00C10L1/04
Inventor 曹蔚王四旺谢艳华康荣杨倩
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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