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Method for building chronic wound animal experiment model

An animal experiment and chronic wound technology, applied in the field of medical research, can solve the problems of no model, unclear pathogenesis and healing mechanism of chronic wound, confusion of researchers, etc., and achieve the effect of simple modeling, high repeatability and stable model.

Inactive Publication Date: 2014-02-19
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

So far, the pathogenesis and healing mechanism of chronic wounds is still unclear, so the research on its mechanism has become a hot and difficult point in the field of wound healing. Most of the previous studies have confused researchers because there is no ideal model

Method used

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  • Method for building chronic wound animal experiment model
  • Method for building chronic wound animal experiment model
  • Method for building chronic wound animal experiment model

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Experimental program
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Embodiment

[0021] Take SD rats fasting for one day, weigh 65mg / Kg, inject STZ (streptozotocin) intraperitoneally, and take tail vein blood to detect blood sugar after 72 hours. If blood sugar is higher than 16.8mmol / L, it is considered as diabetes model Successfully, anesthetized by intraperitoneal injection of 1% sodium pentobarbital.

[0022] 1) Use an electric razor to shave the long hair on the back, depilate with 10% sodium sulfide, make a mark in the middle of the back of the neck, make a longitudinal incision from both marks, the incision width is 2.5cm, and the length is 5cm (see figure 1 ), contralateral suture after hemostasis (see figure 2 , 3 ), forming a skin tube-like structure;

[0023] 2) After 24 hours, the experimental animal was anesthetized, the suture was opened, the tube-like structure was opened and sewed back, and the wound surface was made at the original leather tube-like structure. The blue mark 2*1cm is intended to be the wound area, cut off the marked ski...

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Abstract

Provided is a method for building a chronic wound animal experiment model. The method for building the chronic wound animal experiment model comprises the following steps of (1) selecting a clean experimental animal or building a type II diabetes model in an induction mode, then, shaving off long hair on the back of the experimental animal with an electric shaver, carrying out unhairing with a 10% sodium sulphide solution, making a mark in the center of the nape, making longitudinal cuts in the two sides of the mark, carrying out opposite side suture after hemostasis is carried out, forming a skin-tube-shaped structure, and (2) removing suture lines after 24 hours, opening the skin-tube-shaped structure, suturing the skin back, and making a wound on the original skin-tube-shaped structure. The method for building the chronic wound animal experiment model has the advantages that a modeling method is simple, the model is stable, repeatability is high, and the area is controllable.

Description

technical field [0001] The invention belongs to the technical field of medical research, and in particular relates to a method for constructing a chronic wound animal experimental model. Background technique [0002] Due to the aging population and the gradual increase of diabetes and hypertension, chronic wounds have become one of the diseases with the highest incidence. Chronic wound is a long-term wasting disease, which not only causes great pain to patients, but also brings heavy economic burden to society. So far, the pathogenesis and healing mechanism of chronic wounds is still unclear, so the research on its mechanism has become a hotspot and difficulty in the field of wound healing. In most of the previous studies, researchers were confused because there was no ideal model. Therefore, the establishment of good experimental animal models of chronic wounds is of great significance to the research on the mechanism of chronic wound healing, which will help to understand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
Inventor 白晓智胡大海陶克
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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