In-vitro inflammation model of dairy cow mammary epithelial cells as well as construction method and application of in-vitro inflammation model

A technology of mammary gland epithelial cells and inflammation models, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problem of not being able to completely simulate the actual clinical situation of bacterial infection

Pending Publication Date: 2022-07-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Application Information

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Problems solved by technology

However, LPS is a lipopolysaccharide component in Gram-negative bacteria, which cannot fully simulate the actual clinical situation of bacterial infection

Method used

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  • In-vitro inflammation model of dairy cow mammary epithelial cells as well as construction method and application of in-vitro inflammation model
  • In-vitro inflammation model of dairy cow mammary epithelial cells as well as construction method and application of in-vitro inflammation model
  • In-vitro inflammation model of dairy cow mammary epithelial cells as well as construction method and application of in-vitro inflammation model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Bacterial culture

[0026] The coagulase-negative staphylococcus strains isolated from the milk of clinical mastitis diseased cows were selected and identified as hemolytic staphylococcus GS-1 among the coagulase-negative staphylococci. They were isolated and preserved by Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences. Inoculate in tryptone soybean broth medium, incubate at 37°C, and carry out streak culture on blood plates for later use. Of course, purchased Staphylococcus hemolyticus can also be used.

[0027] Referring to Bergey's Manual of Determinative Bacteriology (Ninth Edition), Coagulase Negative Staphylococcus (CNS) is a type of Staphylococcus negative for plasma coagulase test and positive for Gram staining. Common CNS mainly include Staphylococcus epidermidis , Staphylococcus hemolyticus, Staphylococcus saprophyticus, Staphylococcus ludens, Staphylococcus squirrels, Staphylococcus chromogens, Stap...

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Abstract

The invention provides an in-vitro inflammation model of dairy cow mammary epithelial cells. The in-vitro inflammation model is an injury pathological model of coagulase negative staphylococcus infected dairy cow mammary epithelial cells. According to the invention, cow mammary epithelial cells are taken as a research object, a method for infecting cow mammary epithelial cells with coagulase-negative staphylococcus by taking cow mammary epithelial cells as a carrier is established, an in-vitro inflammation model for simulating bacterial infection of cow mammary cells is established, and proper infection complex number MOI and infection time are determined; and optimizing and determining the detection index of the coagulase-negative staphylococcus infected cow mammary epithelial cells. The model is stable and reliable, and lays a foundation for in-vitro drug screening research on cow mastitis caused by bacteria, especially coagulase-negative staphylococcus, and pathogenic mechanism research on bacteria, especially coagulase-negative staphylococcus.

Description

technical field [0001] The invention relates to an in vitro inflammatory model of dairy cow mammary epithelial cells and a construction method and application thereof. Background technique [0002] Dairy cow mastitis is mainly caused by the invasion of breast tissue by microorganisms or local inflammation caused by physical and chemical stimulation. It has a wide range of occurrence and high morbidity, which seriously threatens the global dairy farming industry and is a worldwide problem. There are many factors that cause mastitis in dairy cows, which are the result of the combined effect of susceptible cattle, pathogenic microorganisms and environmental hygiene, among which bacterial infection is the main cause. [0003] Bacterial infection can cause recessive mastitis and clinical mastitis in dairy cows. Gram-negative bacteria can cause acute mastitis in dairy cows, while Gram-positive bacteria can cause persistent intramammary infection (IMI) in dairy cows, which can run...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/6888G01N33/569G01N33/68C12Q1/02
CPCC12N5/0631C12Q1/6888G01N33/56966G01N33/6869G01N33/6863G01N33/68G01N33/5005C12N2502/70C12N2503/02G01N2333/4721G01N2333/525G01N2333/5412G01N2333/5421G01N2333/545
Inventor 王旭荣王贵波杨峰申纪饶李宏胜张世栋李锦宇张康
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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