Detection method for analyzing xanthium sibiricum adulteration through near infrared spectrum and GBDT algorithm

Abstract

The invention discloses a method applied to rapid detection of certified traditional Chinese medicine xanthium sibiricum and counterfeit xanthium sibiricum doped with substance highly similar characters. The near infrared spectrum of a sample is collected to scan the spectrum of the sample, first derivation, vector normalization, filtering smoothing and other preprocessing are carried out on spectrogram data, principal component information representing the sample to the maximum degree is extracted, and a GBDT algorithm is used for constructing a prediction model for rapidly analyzing certified xanthium sibiricum, adulterated xanthium mongolicum and adulterated Xanthium inaequilatum. The detection method does not need to use a large number of reagents, is green and environment-friendly, simple and convenient to operate, good in reproducibility and stable and accurate in result, can realize rapid online detection and real-time evaluation feedback when the amount of a xanthium sibiricum sample is large, and is beneficial to rapid monitoring and evaluation of xanthium sibiricum quality in the whole process of xanthium sibiricum production, processing, circulation and consumption.

Description

technical field [0001] The invention relates to the technical field of rapid detection methods for traditional Chinese medicine products, in particular to a detection method for analyzing cocklebur adulteration by near-infrared spectroscopy and GBDT algorithm. Background technique [0002] As a common Chinese herbal medicine, cocklebur is the ripe and budded fruit of the dry aerial part of the plant cocklebur of Compositae. Warm in nature, has the effects of dispelling wind and cold, clearing the nose, and dispelling rheumatism. Its chemical composition types are sesquiterpenoids, phenolic acids, flavonoids, anthraquinones, etc., and play anti-tumor, antibacterial, anti-inflammatory, analgesic, hypoglycemic and other pharmacological effects. Cocklebur adulteration often uses Mongolian cocklebur, partial base cocklebur, thorn cocklebur, etc., which belong to the same genus as cocklebur and have a very similar appearance. In particular, the dried stems are taken, crushed into...

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Problems solved by technology

[0003] (1) Character identification: character identification and microscopic identification based on indicators such as size, character, color, thorns, and texture. In view of the wide distribution of cocklebur resources in my country, it is widely distributed in many provinces such as Hebei, Jiangxi, Hunan, Gansu, Qinghai, and Guangdong. The traits of cocklebur are closely related to the climate environment, and the identification of traits is often not accurate enough, and the error is large;

[0004] (2) Physical and chemical inspection and instrument inspection: Thin-layer chromatography, ultraviolet spectroscopy, liquid chromatography, gas chromatography, liquid chromatography-mass spectrometry and other methods are used for detection, which require the use of a large number of organic reagents, which are time-consuming and cumbersome, especially Based on the complexity of traditional Chinese medicine ingredients, it is sometimes difficult to evaluate the integrity of its quality standards for the characteristic response of some specific ingredients

[0008] The highly similar adulterated products from the same genus as Xanthium are difficult to identify in terms of structure by means of microscopic identification and character identification

[0009] (2) It is impossible to evaluate its quality as a whole

[0010] Physical and chemical testing and instrumental testing, aiming at the characteristic response of some specific ingredients, in view of the complexity of the ingredients and efficacy of traditional Chinese medicine, sometimes it is difficult to evaluate the integrity of its quality standards

In particular, samples from different regions in my country and harvested at different times, because of the difference in plant metabolism and environment, their functional chemical components will be different, which is easy to cause errors

[0011] (3) The operation is cumbersome and it is difficult to achieve rapid detection

[0012] The method is often based on complex instrumental analysis, requires the use of a large number of reagents, the pretreatment process is time-consuming and cumbersome, equipped with large analytical instruments and requires operators to have solid analytical experience

Especially in the face of large quantities of cocklebur samples, it is impossible to quickly detect and online monitor and identify their quality, and it is not easy to promote to the majority of small and medium-sized enterprises

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