A kind of preparation method of snake skin active peptide

A technology of active peptides and snake skin, applied in the biological field, can solve the problems of wasting high-quality resources, limiting the utilization efficiency and application range of snake skin, difficult to digest and absorb, and making full use of it

Inactive Publication Date: 2011-12-14
唐作安
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the molecular weight of collagen reaches 30,000-200,000 Dalton, or even larger, it is difficult to be digested and absorbed and fu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Preparation of snake skin active peptide powder with degree of hydrolysis (DH)=15%

[0009] Select 5Kg of fresh snake skins of black snake, red snake, king snake, pine snake, and tiger tail snake, and wash them with clear water; grind them with a meat grinder, and the degree of grinding is controlled by a φ10mm stainless steel orifice plate; grind them with an ultramicro grinder Slurry to a fineness of 20-30μm; put it in a fermenter, add purified water at a ratio of 1:1, heat to 45-55°C, add 537 acid protease to carry out enzymatic hydrolysis at pH 4.0-5.0, 45-50°C For the reaction, the TNBS method was used to control the degree of hydrolysis at 15%, and after the reaction was completed, it was centrifuged and filtered; heated to 90°C and kept for 10 minutes to inactivate the enzyme; polyamide nanofiltration membrane was used for nanofiltration desalination and concentration, and the molecular weight cut-off was 150Dalton; Membrane filtration with a molecular weight cut...

Embodiment 2

[0011] The preparation of the snake skin active peptide powder of degree of hydrolysis (DH)=25%

[0012] Choose each 5Kg of fresh snakeskins of black snake, pine flower snake, king snake, five step snake, and tiger tail snake, and wash them with clear water; grind them with a meat grinder, and the degree of grinding is controlled by a φ8mm stainless steel orifice plate; grind them with an ultrafine grinder Slurry to a fineness of 15-25μm; put it in a fermenter, add purified water at a ratio of 1:1, heat to 50-60°C, add Alcalase alkaline protease to carry out the enzyme under the conditions of pH 8.0-9.0, 50-55°C Hydrolysis reaction, use TNBS method to control the degree of hydrolysis at 25%, centrifuge filtration after the reaction; heat to 95 ° C, keep for 8 minutes to inactivate enzyme; use polyvinyl alcohol nanofiltration membrane for nanofiltration desalination, concentration, molecular weight cut-off adopts 300Dalton; Filtrate with an ultrafiltration membrane, and the mol...

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PUM

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Abstract

The invention relates to a preparation method of snake skin active peptide, in particular to snakes such as black snake, pit viper, red snake, coral snake, cobra, king snake, five step snake, pine snake, tiger tail snake, Chinese water snake, etc. The fresh snake skin is used as the raw material, after cleaning → mincing → refining → adjusting the material concentration → adjusting the material temperature → adding protease enzymatic hydrolysis → centrifugal filtration → deenzyme → nanofiltration desalination concentration → ultrafiltration purification → freeze drying and other technological processes , to obtain snake skin active peptide. More than 90% of the molecular weight of the product is distributed between 200-5000Dalton, of which 200-2000Dalton (2-10 peptide) accounts for more than 75%, and it also contains docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) It has good water solubility, excellent moisture absorption and moisturizing function, good acid and heat stability. It can be used in the fields of medicine and health food production.

Description

technical field [0001] The invention relates to a preparation method of snakeskin active peptide, in particular to hydrolyzing snakeskin protein with biological protease, desalting and concentrating by nanofiltration, purifying by ultrafiltration, and freeze-drying. Belongs to the field of biotechnology. Background technique [0002] Snake skin is usually used to make musical instruments such as Erhu and Jinghu, daily necessities such as bags, backpacks, belts, and leather shoes, as well as high-end handicrafts, as well as delicious food and health food. [0003] Snake skin is rich in nutrients such as collagen, high-quality fat, various minerals, and multiple vitamins, which can repair skin, mucous membranes, blood vessels, ligaments, and bones. Since the molecular weight of collagen reaches 30,000-200,000 Dalton, or even larger, it is difficult to be digested and absorbed and fully utilized. The utilization efficiency and application range of snakeskin are greatly limite...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 唐作安
Owner 唐作安
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