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Primer composition for identifying rice indica subspecies ecotype and its application

A primer composition, ecotype technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc. The advantage prediction effect is not ideal and other problems, to achieve the effect of high sensitivity, shortened identification time, and good resolution

Inactive Publication Date: 2011-12-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ecological types divided according to the ecological conditions in different regions cannot effectively reflect the genetic differences between parents, and the prediction effect on heterosis is not ideal
In addition, the use of heterosis between indica and japonica rice subspecies has been widely concerned. However, due to many problems such as semi-sterility in the F1 generation, it has been difficult to directly use heterosis between subspecies. The utilization of heterosis has become the consensus among breeders
However, there are few studies on reasonable and feasible division of ecotypes within subspecies

Method used

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  • Primer composition for identifying rice indica subspecies ecotype and its application
  • Primer composition for identifying rice indica subspecies ecotype and its application
  • Primer composition for identifying rice indica subspecies ecotype and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, the preparation of primer composition

[0047] The nucleotide sequences of each primer are as follows:

[0048] rm216 primer pair (annealing temperature is 48°C):

[0049] Upstream primer: 5'-gcatggccgatggtaaag-3' (sequence 1 of the sequence listing);

[0050] Downstream primer: 5'-tgtataaaaccacacggcca-3' (sequence 2 of the sequence listing);

[0051] rm225 primer pair (annealing temperature is 48°C):

[0052] Upstream primer: 5'-tgcccatatggtctggatg-3' (sequence 3 of the sequence listing);

[0053] Downstream primer: 5'-gaaagtggatcaggaaggc-3' (sequence 4 of the sequence listing);

[0054] rm235 primer pair (annealing temperature is 50°C):

[0055] Upstream primer: 5'-agaagctagggctaacgaac-3' (sequence 5 of the sequence listing);

[0056] Downstream primer: 5'-tcacctggtcagcctctttc-3' (sequence 6 of the sequence listing);

[0057] rm25 primer pair (annealing temperature is 50°C):

[0058] Upstream primer: 5'-ggaaagaatgatcttttcatgg-3' (sequence 7 of th...

Embodiment 2

[0082] Embodiment 2, the application of primer composition

[0083] The 428 rice materials belong to the indica subspecies and were all obtained from the China National Crop Germplasm Bank. icgr.caas.net.cn / ).

[0084] Zhang Dongling et al. (2009) used SSR markers and the primary core germplasm of Chinese rice seed resources to analyze the genetic structure and geographical evolution, and divided the indica subspecies into three ecotypes: early indica ecotype, late indica ecotype and intermediate ecotype (Zhang Dongling, Zhang Hongliang, Wang Meixing, Sun Junli, Qi Yongwen, Wang Fengmei, Wei Xinghua, Han Longzhi, Wang Xiangkun, Li Zichao. The Hierarchical Genetic Structure and Differentiation of Oryza sativa L. in China Revealed by Microsatellites. Theor Appl Genet 2009116 (6): 1105-1117). According to the classification standard in the literature, among the 428 rice materials, there were 157 early indica eco-type varieties, 99 intermediate indica eco-type varieties, and 172...

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Abstract

The invention discloses a primer composition for identifying the ecotype of indica subspecies of rice and use thereof. The primer composition disclosed by the invention comprises the following primer pairs: a primer pair rm 216 consisting of DNAs represented by sequences 1 and 2, a primer pair rm225 consisting of DNAs represented by sequences 3 and 4, a primer pair rm235 consisting of DNAs represented by sequences 5 and 6, a primer pair rm25 consisting of DNAs represented by sequences 7 and 8, a primer pair rm 23 consisting of DNAs represented by the sequences 9 and 10, a primer pair rm 244 consisting of DNAs represented by sequences 11 and 12, a primer pair rm 253 consisting of DNAs represented by sequences 13 and 14, a primer pair rm 267 consisting of DNAs represented by sequences 15 and 16, a primer pair rm 296 consisting of DNAs represented by sequences 17 and 18, a primer pair rm 223 consisting of DNAs represented by sequences 19 and 20 and a primer pair rm 258 consisting of DNAsrepresented by sequences 21 and 22. The primer composition discloses by the invention can be used for identifying the ecotype of the indica subspecies of rice, has the advantages of quickness, convenience, high efficiency, high sensitivity, high identifying capacity, accurate and reliable result and the like.

Description

technical field [0001] The invention relates to a primer composition for identifying rice indica subspecies ecotype and application thereof. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world. As one of the origins of cultivated rice in Asia (Oka 1988), China has abundant rice seed resources. At present, the National Germplasm Bank has collected and preserved more than 70,000 accessions of cultivated rice, of which indica germplasm resources account for 67% (ICGR CAAS 1996). The planting area of ​​indica rice in my country accounts for 70% of the total area of ​​rice, and indica rice is the rice with the highest degree of hybridization, and the planting area of ​​hybrid indica rice has accounted for nearly 80% of the area of ​​indica rice in China. Therefore, indica rice plays an important role in rice research and production practice in my country. [0003] The classification of cultivated rice has always been highly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李自超张冬玲张洪亮王美兴王凤梅宋立莹马占峰
Owner CHINA AGRI UNIV
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