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Bacillus thuringiensis with activity on resisting Bacillus thuringiensis (Bt) plutella xylostella

A technology of Bacillus thuringiensis and Bacillus aureus, applied in bacteria, microorganism-based methods, biocides, etc., can solve problems such as ineffectiveness, and achieve good results, good application and promotion prospects, and good economic effects.

Active Publication Date: 2012-01-25
哈尔滨微华生物科技有限公司
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AI Technical Summary

Problems solved by technology

These bacteria produce one or more insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs, also known as δ-endotoxins) during the spore formation period, which can be effective against Lepidoptera, Coleoptera, Hemiptera, Diptera , Homoptera, Hymenoptera, Orthoptera, Isoptera, Trichoptera, Neuroptera, Flea, Thysanoptera, Trichophaera, Blattata, Acarids, Lice, Trematodes, Tapeworms, Various insects and protists such as flagellates, amoebas and paramecia produce toxin activity, but have no effect on non-target organisms such as humans and animals, and are environmentally friendly and safe

Method used

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specific Embodiment approach 1

[0016] Embodiment 1: In this embodiment, a strain of Bacillus thuringiensis active against Bt diamondback moth is Bacillus thuringiensis AH-2, which belongs to the genus Bacillus and has been managed by the preservation of microbial strains in China. Deposited by the General Microbiology Center of the Committee, the deposit address is No. 1 Beichen West Road, Chaoyang District, Beijing. The Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No: 5220, and the deposit date is September 6, 2011; it is Gram-positive Aerobic bacteria, 3.2 μm to 4.8 μm in length and 1.2 μm to 1.8 μm in width, one vegetative cell has only one spore, the spore is mesophytic, subterminal or terminal, the sporangia do not expand, and the parasporal crystals are diamond-shaped, There are flagella; white round colonies are formed on beef extract peptone medium, the colonies are flat, the surface is dry and dull, and the edges are notched.

[0017] In this embodiment, Bacil...

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Abstract

The invention discloses a Bacillus thuringiensis with activity on resisting Bacillus thuringiensis (Bt) plutella xylostella, and relates to a Bacillus thuringiensi. The Bacillus thuringiensis is Bacillus thuringiensis AH-2 and belongs to the Bacillus, and is preserved in the China General Microbiological Culture Collection Center with the collection number of CGMCC No: 5220 in September 6, 2011. The Bacillus thuringiensis AH-2 has strong action on poisoning and killing Lepidoptera and Coleoptera main pests and resisting Bt and Cry1Ac toxalbumin pests. The Bacillus thuringiensis can be used for producing efficient broad-spectrum insecticide, can be used for preventing the Lepidoptera important pests such as plutella xylostella and the Coleoptera pest such as yellow mealworm, has good effect on resisting pests and is favorable for preventing and controlling field resistance plutella xylostella.

Description

technical field [0001] The invention relates to a strain of Bacillus thuringiensis. Background technique [0002] Bacillus thuringiensis (Bt) is a Gram-positive bacterium isolated from soil or from dead insects. These bacteria produce one or more insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs, also known as δ-endotoxins) during the spore formation period, which can be effective against Lepidoptera, Coleoptera, Hemiptera, Diptera , Homoptera, Hymenoptera, Orthoptera, Isoptera, Trichoptera, Neuroptera, Flea, Thysanoptera, Trichophaera, Blattata, Acarids, Lice, Trematodes, Tapeworms, Various insects and protists such as flagellates, amoebas and paramecia produce toxin activity, but they have no effect on non-target organisms such as humans and animals, and are environmentally friendly and safe. Therefore, Bacillus thuringiensis is widely used as the main biological pesticide. The inoculum has been used as the main component of spray-type insecticides for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/07
Inventor 杨峰山朱勋张友军李云龙刘春光杨晶郑晓旭薛原滕淼
Owner 哈尔滨微华生物科技有限公司
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