Medicament composition containing antisense polynucleotide targeting miR-214

An antisense polynucleotide and polynucleotide technology, applied in the field of antisense nucleic acid pharmaceutical compositions, can solve the problems that there are no literature reports on the role of miR-214

Active Publication Date: 2013-01-30
SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The above research results show that miR-214 plays an important role in the regulation of bone development, but there is no literature reporting the role of miR-214 in the regulation of osteoblast function

Method used

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  • Medicament composition containing antisense polynucleotide targeting miR-214
  • Medicament composition containing antisense polynucleotide targeting miR-214
  • Medicament composition containing antisense polynucleotide targeting miR-214

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Embodiment

[0075] Hereafter, although an Example demonstrates this invention more concretely, this invention is not limited to these Examples.

[0076] 1. After MC3T3-E1 cells were transfected with antisense nucleic acids with different degrees of complementarity to miR-214, the inhibition efficiency of intracellular miR-214. 1# The oligonucleotide that is completely complementary to miR-214; 2# The 3' end of the antisense nucleic acid has a 1 base mutation; 3# The 3' end of the antisense nucleic acid has a 13 base mutation; 4# There are 6 base mutations at the 3' end of the antisense nucleic acid; 5 base mutations at the 3' end of 5# antisense nucleic acid; 1 base mutation at the 5' end of 6# antisense nucleic acid; 7 #There is another base mutation at the 5' end of the antisense nucleic acid; the ribose in the above oligonucleotide is modified with 2-methoxy (2'-OMe) to improve the stability of siRNA. After the above oligonucleotides were transfected with Lipofectamin2000 (Invitrogen)...

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Abstract

The invention relates to a medicament composition containing antisense polynucleotide targeting miR-214 and a pharmaceutical purpose of the antisense polynucleotide. The medicament composition contains the antisense polynucleotide targeting miR-214 as an active component and further contains a carrier acceptable for medicaments.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine, and in particular relates to an antisense nucleic acid pharmaceutical composition and pharmaceutical application of the antisense nucleic acid. Background technique [0002] Osteoblasts are a mesenchymal-derived cell type that play an important role in bone development and bone formation. A deep understanding of the regulatory mechanisms of osteoblast function is a prerequisite for combating bone loss such as osteoporosis. In the last two decades, advances in molecular and genetic research have revealed various regulatory processes during osteoblast differentiation. Central to these regulations are transcription factors. RunX2, Osterix, and β-catenine are essential transcription factors during osteoblast differentiation. Some other transcription factors C / EBPβ, Smad1 and Smad5 combined with RunX2 to enhance its transcriptional activity. However, Twist1 can repress the transcript...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61P19/08A61P19/10A61K48/00
Inventor 李英贤王晓刚李琦李莹辉白延强陈善广
Owner SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
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