A blood test kit for the diagnosis of osteoporosis

A technology for blood detection and osteoporosis, applied in the field of blood detection kits, can solve problems such as reducing protein level and mRNA, and achieve the effect of improving sensitivity, convenient diagnosis and treatment

Active Publication Date: 2017-11-14
SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These small RNA molecules do not encode proteins, but they reduce protein levels in vivo by reducing mRNA levels or binding to mRNA 3'UTR to inhibit translation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A blood test kit for the diagnosis of osteoporosis
  • A blood test kit for the diagnosis of osteoporosis
  • A blood test kit for the diagnosis of osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Correlation between the level of miR-214 in serum and the level of miR-214 in bone tissue

[0059] Osteoblast-specific transgenic mice were constructed, microRNA in bone tissue and serum were extracted, and the content of miR-214 was determined by Q-PCR. The results showed that there was no correlation between the level of miR-214 in serum and the level of miR-214 in bone tissue. The specific method is as follows:

[0060] Construction of osteoblast-specific transgenic mice:

[0061] Using the OCN promoter specifically expressed in osteoblasts to drive the expression of miRNA-214, the vector directly injects foreign genes into fertilized eggs, so that the foreign genes can be integrated into the DNA to form osteoblast-specific transgenic animals, that is, miR-214 in Osteoblast-specific high expression.

[0062] Extraction of bone tissue miRNA:

[0063] Identify osteogenesis-specific transgene-positive mice and littermate control wild-type mice, anesthetiz...

Embodiment 2

[0088] Example 2. Correlation between the level of miR-214 in serum and the T value of lumbar bone mineral density

[0089] Extraction and detection of miR-214 in patient serum:

[0090] Same as Example 1

[0091] Detection of lumbar bone density in patients:

[0092]Using dual-energy X-ray absorptiometry, the bone density of the lumbar spine (Ll-14) of the patients was measured, and the results were standardized, and the T value was calculated by the bone absorptiometry, and then grouped and scored accordingly.

[0093] Statistical Analysis:

[0094] The correlation analysis method in GraphPad Prim5.1 can be used to verify the linear relationship between the level of miR-214 in serum and the T value of lumbar spine bone density. From the correlation coefficient r, we can know whether the two variables are linearly related or not. strength, and whether it is positively or negatively correlated.

[0095] Results: The level of miR-214 in serum was significantly negatively co...

Embodiment 3

[0096] Example 3. Correlation between the level of miR-214 in serum and the amount of carboxy-terminal (CTX) type I collagen, a sensitive indicator of bone resorption rate

[0097] Extraction and detection of miR-214 in patient serum:

[0098] Same as Example 1

[0099] Detection of carboxy-terminal (CTX) type I collagen, a sensitive indicator of bone resorption rate in patient serum:

[0100] The content of carboxy-terminal (CTX) of type I collagen in serum was detected by Elisa method.

[0101] Statistical Analysis:

[0102] The correlation analysis method in GraphPad Prim5.1 can be used to verify the linear relationship between the level of miR-214 in serum and the CTX value. From the correlation coefficient r, we can know whether the two variables have a linear relationship, the strength of the linear relationship, and Is it positively or negatively correlated

[0103] result:

[0104] The level of miR-214 in serum was significantly positively correlated with the amou...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a blood detection kit for osteoporosis diagnosis, which comprises primers for amplifying miR-214. Besides, the invention also relates to application of the primers for amplifying miR-214 in preparing the blood detection kit for osteoporosis diagnosis.

Description

[0001] This application claims the priority of Chinese invention patent application 201410050513.3 (application date: February 14, 2014, invention name: a blood test kit for auxiliary diagnosis of osteoporosis). technical field [0002] The invention belongs to the field of molecular diagnosis, and in particular relates to a blood detection kit which can be used for the diagnosis of osteoporosis. In addition, the present invention also relates to the use of primers for amplifying miR-214 in the preparation of a blood test kit that can be used for the diagnosis of osteoporosis. Background technique [0003] Osteoporosis refers to a skeletal disease characterized by lower than normal bone mass per unit volume. Insufficient production of bone organic components, secondary calcium salt reduction, and micropore structure destruction of bone tissue are the main manifestations. Osteoporosis has become a worldwide problem, and it is another disease that seriously endangers human hea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 李英贤凌树宽李琦
Owner SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap