Application of miR-214 to preparation of product for preventing or treating alzheimer disease

A mir-214, 1. The technology of mir-214 is applied in the field of application of miR-214 in the preparation of products for the prevention or treatment of Alzheimer's disease, which can solve the problems of unexplained etiology and pathogenesis

Inactive Publication Date: 2013-04-10
PEKING UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

The etiology and pathogenesis of

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  • Application of miR-214 to preparation of product for preventing or treating alzheimer disease
  • Application of miR-214 to preparation of product for preventing or treating alzheimer disease
  • Application of miR-214 to preparation of product for preventing or treating alzheimer disease

Examples

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Embodiment 1

[0032] Example 1, the application of miR-214 in the prevention or treatment of Alzheimer's disease

[0033] 1. Establishment of Alzheimer's neuron cell model

[0034] Amyloid-beta treatment of isolated neurons well mimics Alzheimer's disease as follows:

[0035] 1. Obtain neuronal cells by isolation and culture

[0036] 1) Coating: Coat the culture bottle (plate) with poly-lysine for 10 minutes before the culture, suck it off, dry it naturally on the sterile operating table for 15 minutes, and put it in the incubator for standby;

[0037] 2) Brain extraction: Select several newborn 24-hour SD rats (Weitong Lihua, name: SD rat 24-hour suckling mouse), male or female, disinfect the whole body with 75% alcohol, decapitate, and execute under aseptic conditions Cut the scalp and skull in layers, use curved tweezers to open the view of the brain area, carefully remove the whole brain, and wash it with D-hanks solution for several times; (Prepare at least 4 pairs of ophthalmic scis...

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Abstract

The invention discloses an application of miR-214 (microRNA) to preparation of a product for preventing or treating an alzheimer disease, and provides an application of the miR-214 to preparation of 1) a product for preventing and/or treating the alzheimer disease, and/or 2) a product for reducing the neuron cell death quantity of the alzheimer disease. A nucleotide sequence of the miR-214 is Sequence 1 as shown in a sequence table. An experiment of the application proves that neurodegenerative diseases, such as the alzheimer disease, can be prevented and/or treated through improving substances expressed by the miR-214. In clinical diagnosis at an earlier stage, the miR-214 can also serve as a biological index for diagnosing the neurodegenerative diseases, and a doctor can perform early diagnosis of the neurodegenerative diseases timely on a patient through detecting an miR-214 level in the patient and combining with other effective indexes, and further effectively controls the neurodegenerative diseases and the loss of a cognition level.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of miR-214 in the preparation of products for preventing or treating senile dementia. Background technique [0002] Senile dementia (Alzheimer, AD) is a degenerative disease of the central nervous system, with an insidious onset and a chronic progressive course. It is the most common type of senile dementia. The main manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language barriers, which seriously affect social, occupational, and life functions. The etiology and pathogenesis of AD have not yet been elucidated. Alzheimer's is a degenerative disease that cannot be detected until autopsy. Contents of the invention [0003] One object of the present invention is to provide new uses of miR-214. [0004] The present invention provides the application of miR-214 in the preparation of the...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P25/28
Inventor 张研
Owner PEKING UNIV
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