Fish-skin degreasing method

A fish skin and lipase technology, applied in application, food preparation, food science and other directions, can solve the problems of poor production safety, large amount of use, unsuitable for industrial production, etc., and achieve the effect of mild method and simple process

Inactive Publication Date: 2012-03-07
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that the isopropanol used is a volatile flammable organic solvent, which has a large amount of use and poor production safety, and is not suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for degreasing fish skin, the concrete steps are as follows:

[0029] Use the commercially available tilapia skin as raw material, soak it in deionized water for 8 hours, and then wash it with deionized water. The amount of deionized water used is 8 times the weight of the fish skin, and then cut the cleaned fish skin with scissors. Grow into 1cm squares and set aside.

[0030] Add the shredded fish skin to an alkaline lipase enzyme solution with an activity of 50U / mL and a pH value of 8.0 at a weight ratio of 1:4 for enzymolysis treatment. The enzymolysis temperature is 37°C. After stirring for 25 minutes, the temperature is raised to Inactivate the enzyme at 80°C, then cool to room temperature and filter.

[0031] Add 5 times the volume of aqueous sodium hydroxide solution with a pH value of 7.5 to the filtrate obtained by enzymolysis, stir for 15 minutes and then filter, repeating 3 times. Then, add 8 times the volume of deionized water to the filtrate obt...

Embodiment 2

[0034] A method for degreasing fish skin, the concrete steps are as follows:

[0035] Take the commercially available tilapia skin as raw material, soak it in deionized water for 8 hours, and then wash it with deionized water. The amount of deionized water used is 8 times the weight of the fish skin, and then cut the cleaned fish skin with scissors. Grow into 1cm squares and set aside.

[0036] Add the shredded fish skin to an alkaline lipase enzyme solution with an activity of 60U / mL and a pH value of 8.0 at a weight ratio of 1:5 for enzymolysis treatment. The enzymolysis temperature is 35°C. After stirring for 55 minutes, the temperature is raised to Inactivate the enzyme at 60°C, then cool to room temperature and filter.

[0037] Add 5 times the volume of aqueous sodium hydroxide solution with a pH value of 8.0 to the filtrate obtained by enzymatic hydrolysis, stir for 20 minutes and then filter, repeating 3 times. Then, add 8 times the volume of deionized water to the fi...

Embodiment 3

[0040] A method for degreasing fish skin, the concrete steps are as follows:

[0041] Take the commercially available tilapia skin as raw material, soak it in deionized water for 8 hours, and then wash it with deionized water. The amount of deionized water used is 8 times the weight of the fish skin, and then cut the cleaned fish skin with scissors. Grow into 1cm squares and set aside.

[0042] Add the shredded fish skin to an alkaline lipase enzyme solution with an activity of 80U / mL and a pH value of 8.0 at a feed-to-liquid weight ratio of 1:6 for enzymolysis treatment. The enzymolysis temperature is 33°C. After stirring for 85 minutes, the temperature is raised to Inactivate the enzyme at 60°C, then cool to room temperature and filter.

[0043] Add 5 times the volume of aqueous sodium hydroxide solution with a pH value of 8.5 to the filtrate obtained by enzymatic hydrolysis, stir for 25 minutes and then filter, repeating 3 times. Then, add 8 times the volume of deionized ...

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PUM

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Abstract

The invention belongs to the field of aquatic product processing and specifically relates to a fish-skin degreasing method, which comprises the following steps of: immersing fish-skin, cutting into pieces, adding an alkaline lipase enzyme preparation which has pH value of 8.0 and vitality of 50-80U / mL and is 4-6 times as heavy as the fish-skin, carrying out enzymatic hydrolysis at 35+ / -2 DEG C for 25-85 min, heating up to 60-80 DEG C for killing enzyme, cooling to room temperature, filtering, washing off aliphatic acid and residual fat generated from enzymatic hydrolysis by using alkali lye, and washing off alkali lye and other impurities by using deionized water. The invention has advantages of simple technology, mild condition and high degreasing rate, and is suitable for expansion and mass production.

Description

technical field [0001] The invention belongs to the field of aquatic product processing, and in particular relates to a method for degreasing fish skin by combining an enzymatic method and a water bleaching method. Background technique [0002] Fish skin is a high value-added by-product of aquatic product processing. It is rich in active substances such as collagen and mucopolysaccharides, and is a good raw material for the production of gelatin beauty and health care medicines. Because the fat in fish skin is easy to oxidize and rot, producing peculiar smell, degreasing is an essential process in the high-value utilization of fish skin. [0003] At present, solvent method, water bleaching method and enzymatic method are generally used for degreasing. The solvent method uses the principle of similar compatibility, and generally uses organic solvents such as n-hexane, isopropanol, ethyl acetate, and ethanol to achieve the purpose of degreasing, and organic solvents are poiso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/325A23L1/015A23L17/00A23L5/20
Inventor 廖妙飞郑斌杨会成付万冬钟明杰傅光明
Owner ZHEJIANG MARINE DEV RES INST
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