Analysis method for activity of mismatch repair of zebra fish embryo DNA and application thereof
A technology of zebrafish embryos and analysis methods, applied in the analysis of DNA mismatch repair function activity of zebrafish embryos, and the application field of environmental compound DNA mismatch repair function toxicity evaluation, achieving less dosage, easy feeding, and small size Effect
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[0036] It should be pointed out that the following specific descriptions are all exemplary, and are intended to provide further description of the present invention. Unless defined otherwise, all scientific and technical terms used herein have the same meaning as commonly understood by one skilled in the art to which this invention belongs.
[0037] The present invention will be further described below in conjunction with specific examples.
[0038] Below in conjunction with accompanying drawing for further explanation:
[0039] figure 1 Shown is the analysis method of zebrafish embryo DNA MMR activity, and the specific implementation steps are as follows.
[0040] 1. Obtaining of single-stranded DNA molecules (see flow chart figure 2 )
[0041] A. Use the helper phage M13ko7 to infect UltraMax DH5α / p111 engineering bacteria to prepare ssDNA (M13KO7 was purchased from Beijing NEB Company), where pGEM-EGFP is p111, and pGEM-(mt)EGFP is p189:
[0042] 1) One day in advance...
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