Extraction method for total RNA of feed type tetraploid robinia pseudoacacia leaves
A tetraploid Robinia pseudoacacia extraction method technology, applied in the field of plant molecular biology, can solve the problems of inability to extract high-quality RNA, difficult to effectively remove or inhibit, and unstable products, so as to reduce operating time and material consumption, Save operating time and cost, and easy to operate
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[0026] The general idea of the present invention is: based on common equipment and domestic chemical reagents, with the goal of eliminating the interference of protein and polysaccharide, it adopts the method of adding bentonite in the extract, adding a large amount of potassium acetate before precipitation, etc. to remove excessive protein and polysaccharide The key technology to obtain complete and stable RNA products. The specific method is: directly grind the leaves of Robinia pseudoacacia into powder in a centrifuge tube, add ice-bath buffer and β-mercaptoethanol, and centrifuge; discard the supernatant, add extraction buffer and β-mercaptoethanol, oscillate and mix, heat Water bath treatment; add water-saturated phenol and chloroform, oscillate to mix, and centrifuge; absorb the supernatant, add anhydrous ethanol and potassium acetate solution to mix, put in an ice bath and then centrifuge, discard the supernatant, and wash with 70% ethanol After centrifugation, the su...
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