Sensitive, accurate and visual method for detecting antimicrobial peptide products and suitable for industrial production

Abstract

The invention provides a method for detecting an antimicrobial peptide product and suitable for industrial production. The method comprises the following steps of: (1) preparing a culture medium for detection; (2) preparing test bacteria; (3) preparing a solution of the antimicrobial peptide product; (4) setting up a standard curve; (5) performing quality inspection; and (6) determining test results. The method can detect antimicrobial activity of the antimicrobial peptide product sensitively, accurately and visually, is simple and convenient in operation, needs to make only one standard curve under the same production process conditions, makes the results stable and accurate, can provide the detection results in the form of precise numbers, can effectively ensure that the experimental data is true and reliable, and is suitable for large-scale production testing.

Description

technical field [0001] The invention relates to a detection method of an antibacterial peptide product. Background technique [0002] The antimicrobial peptides that have appeared in recent years are a new type of antibacterial drugs with great development potential. They are small molecule cationic peptides produced by the host to resist external pathogen infection. In addition to anti-bacteria, fungi and viruses and other pathogens, it also has anti-tumor cell effects. Because their antibacterial function was first discovered, they were named antimicrobial peptides (AMPs). The broad-spectrum antibacterial activity and multiple immune functions of antimicrobial peptides make it not only used as a feed additive to feed animals to prevent diseases, but also as an injection to treat various infectious diseases; at the same time, as a natural green product of organisms, it can also be used in molecular biology Technology and genetic engineering means to introduce its gene int...

AI Technical Summary

Problems solved by technology

This method solves the shortcomings of existing detection methods

Images