Shrimp larvae microinjection method
A technology of microinjection and shrimp fry, applied in fish farming, application, climate change adaptation, etc., can solve the problems of numerous components, difficult to carry, injection, etc., and achieves simple operation method, high injection success rate, and injection wound. small effect
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[0013] A microinjection method for shrimp seedlings, the specific steps comprising:
[0014] Step (1). Take the exogenous injection sample, and draw the quantitative liquid into the microinjection needle of the nanoliter microsyringe by using a manual micromanipulator and a nanoliter microsyringe;
[0015] Step (2). Place the shrimp on a horizontal platform, absorb the moisture outside the carapace, then place the shrimp in a petri dish covered with gauze, with the belly of the shrimp facing upwards, and gently press the forehead of the carapace As well as the tail of the shrimp body, fix the shrimp body to prevent it from bouncing, move the petri dish to the field of view of the microscope, and adjust the focal length to make the image of the injected animal clear;
[0016] Step (3). Adjust the needle of the microinjection needle to enter the field of view of the microscope, and make sure that the needle and the shrimp body are in the same field of view of the stereomicroscop...
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