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Shrimp larvae microinjection method

A technology of microinjection and shrimp fry, applied in fish farming, application, climate change adaptation, etc., can solve the problems of numerous components, difficult to carry, injection, etc., and achieves simple operation method, high injection success rate, and injection wound. small effect

Inactive Publication Date: 2013-05-01
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the entire microinjection device has many components and precise equipment, is not easy to carry around, and cannot be directly used for in vivo injection of living shrimp larvae.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] A microinjection method for shrimp seedlings, the specific steps comprising:

[0014] Step (1). Take the exogenous injection sample, and draw the quantitative liquid into the microinjection needle of the nanoliter microsyringe by using a manual micromanipulator and a nanoliter microsyringe;

[0015] Step (2). Place the shrimp on a horizontal platform, absorb the moisture outside the carapace, then place the shrimp in a petri dish covered with gauze, with the belly of the shrimp facing upwards, and gently press the forehead of the carapace As well as the tail of the shrimp body, fix the shrimp body to prevent it from bouncing, move the petri dish to the field of view of the microscope, and adjust the focal length to make the image of the injected animal clear;

[0016] Step (3). Adjust the needle of the microinjection needle to enter the field of view of the microscope, and make sure that the needle and the shrimp body are in the same field of view of the stereomicroscop...

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PUM

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Abstract

The invention relates to a shrimp larvae microinjection method. A puncture wound area of the present injection method is large, so individual animal is easy to die after being injected. The shrimp larvae microinjection method is characterized in that: a hand-operated micro-operation instrument and a nanoliter microinjector are firstly adopted, and quantitative liquid is absorbed into a microinjection needle; then the shrimp larvae is arranged inside a culture dish having a gauze, the culture dish is moved to the vision of a microscope, by adjusting a pinhead of the microinjection needle, the vision of the microscope can be entered, the position of the microinjection needle is adjusted through the hand-operated micro-operation instrument, a peritoneum hypodermic injection puncture point isdetermined, and the pinhead of the microinjection needle enters the peritoneum hypodermic tissue to perform the microinjection of medicine inside the human body; and after the injection is conformed to finish, the microinjection needle is remained for 1 to 3 seconds, and then the microinjection needle is withdrawn; and finally the culture dish is horizontally moved out of the vision of the microscope, and the shrimp larvae is returned into the water. The method is simple to operate, has small wound area and high injection successful rate.

Description

technical field [0001] The invention relates to a microinjection method, in particular to a method for completing microvolume injection of young shrimp seedlings under a microscope. Background technique [0002] Shrimp belongs to arthropod crustaceans, with long and flat body, divided into head, chest and abdomen, with calcareous exoskeleton on the body surface, and the carapace is hard and thick. In the field of aquaculture research, it is often necessary to quickly and easily inject exogenous substances (such as exogenous genes, DNA fragments, RNA fragments and proteins, etc.) into shrimp. However, shrimps are relatively small, especially the newly hatched shrimps, whose body length is only a few millimeters. The shell is lubricated, lively and active, and they bounce quickly, so it is difficult to capture and inject them. [0003] At present, commonly used disposable syringe models and specifications generally use milliliter-level syringes. For individual shrimp seedlin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 马文明杨卫军俞炎琴肖金星钱叶青
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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