Shrimp larvae microinjection method

Abstract

The invention relates to a shrimp larvae microinjection method. A puncture wound area of the present injection method is large, so individual animal is easy to die after being injected. The shrimp larvae microinjection method is characterized in that: a hand-operated micro-operation instrument and a nanoliter microinjector are firstly adopted, and quantitative liquid is absorbed into a microinjection needle; then the shrimp larvae is arranged inside a culture dish having a gauze, the culture dish is moved to the vision of a microscope, by adjusting a pinhead of the microinjection needle, the vision of the microscope can be entered, the position of the microinjection needle is adjusted through the hand-operated micro-operation instrument, a peritoneum hypodermic injection puncture point isdetermined, and the pinhead of the microinjection needle enters the peritoneum hypodermic tissue to perform the microinjection of medicine inside the human body; and after the injection is conformed to finish, the microinjection needle is remained for 1 to 3 seconds, and then the microinjection needle is withdrawn; and finally the culture dish is horizontally moved out of the vision of the microscope, and the shrimp larvae is returned into the water. The method is simple to operate, has small wound area and high injection successful rate.

Description

technical field [0001] The invention relates to a microinjection method, in particular to a method for completing microvolume injection of young shrimp seedlings under a microscope. Background technique [0002] Shrimp belongs to arthropod crustaceans, with long and flat body, divided into head, chest and abdomen, with calcareous exoskeleton on the body surface, and the carapace is hard and thick. In the field of aquaculture research, it is often necessary to quickly and easily inject exogenous substances (such as exogenous genes, DNA fragments, RNA fragments and proteins, etc.) into shrimp. However, shrimps are relatively small, especially the newly hatched shrimps, whose body length is only a few millimeters. The shell is lubricated, lively and active, and they bounce quickly, so it is difficult to capture and inject them. [0003] At present, commonly used disposable syringe models and specifications generally use milliliter-level syringes. For individual shrimp seedlin...

AI Technical Summary

Problems solved by technology

However, the entire microinjection device has many components and precise equipment, is not easy to carry around, and cannot be directly used for in vivo injection of living shrimp larvae.