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Microbial fertilizer containing bacillus subtilis

A technology of Bacillus subtilis and microbial fertilizers, applied in the direction of organic fertilizers, can solve environmental pollution and other problems, and achieve the effects of preventing plant death, good therapeutic effect, and improving utilization efficiency

Inactive Publication Date: 2012-06-27
THE IT ELECTRONICS ELEVENTH DESIGN & RES INST SCI & TECHNOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This shows that it is limited to increase crop yields only by increasing the application of chemical fertilizers in large quantities, not to mention a series of problems such as environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Trichoderma korningii

[0066] Conventional potato plus agar medium, cultivated at 25-27°C for 24-48h.

[0067] secondary medium

[0068] Remove the agar and incubate at 25-27°C for 48-72h.

[0069] Tertiary training

[0070] The composition of the medium is calculated by mass percentage: 0.5% sodium acetate, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium carbonate, and the balance is water. Culture at 25-27°C for 2-5 days.

[0071] Bacillus subtilis

[0072] First level training

[0073] Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, and the balance is water. PH 7-7.2, temperature 28°C~35°C, culture time 5~7 days.

[0074] Secondary training:

[0075] The agar is removed from the primary medium, the pH is 7-7.2, the temperature is 28°C~35°C, and the culture time is 5~7 days.

[0076] Ter...

Embodiment 2

[0079] Photosynthetic bacteria

[0080] First level training

[0081] PH6~10, temperature 26-35℃, light intensity 3000-4000 lux, time 6-8 days

[0082] Secondary training

[0083] culture conditions same level culture

[0084] Tertiary training

[0085] Inoculate the secondary cultured bacteria into the seed tank, pH 7~8, temperature 26-35°C, light intensity 3000-4000 lux, for 6-8 days; then inoculate in the fermenter for 2-3 days, and the culture conditions are the same as above.

[0086] primary medium

[0087] The composition of the medium is calculated by mass percentage: 0.5% yeast extract, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium carbonate, and the balance is water. Adjust the pH value to 7.1-7.2, the fermentation culture temperature is 28°C~35°C, and the culture time is 20~24h;

[0088] secondary medium

[0089] The composition of the medium is calculated by mass percentage: 0.5% yeast extrac...

Embodiment 3

[0093] Actinomycetes

[0094] First level training

[0095] Medium: 1.5% sucrose, 0.05% potassium dihydrogen phosphate, 0.05% calcium carbonate, 0.05% magnesium sulfate, 0.02% sodium chloride, 0.05% yeast extract, 3% agar. PH6~8, temperature 28-32℃, time 5-7 days.

[0096] Secondary training

[0097] The agar is removed from the first-level medium, and the culture conditions are the same as that of the first-level culture.

[0098] Three-level training:

[0099] Medium weight volume ratio: glucose 1-3%, yeast juice 1-2%, potassium dihydrogen phosphate 0.01-0.05%, sodium chloride 0.02-0.05%, ferrous sulfate 0.001-0.005%, the balance is water, PH7 .2-7.5, temperature 28-32 ℃, time 5-7 days.

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Abstract

The invention discloses a microbial fertilizer, which comprises a microbial zymotic fluid consisting of photosynthetic bacteria, lactic acid bacteria, actinomyces, Corning trichoderma and a bacillus subtilis zymotic fluid in the volume ratio of 1:(0.8-1):(1.2-1.4):(0.6-0.8):(1-1.2). The utilization efficiency of the fertilizer prepared with the method can be increased, and the effects of preventing withering and plant diseases of plants can be achieved.

Description

technical field [0001] The invention relates to a microbial fertilizer and belongs to the field of planting. Background technique [0002] Microbial fertilizer is a product in which the life activities of microorganisms cause crops to obtain specific fertilizer effects, and it is a type of fertilizer used in agricultural production. It has a history of nearly 50 years in China. Microbial fertilizer is a living fertilizer, and its function is mainly completed by the life activities of a large number of beneficial microorganisms it contains. Only when the life activities of these beneficial microorganisms are completed. Only when these beneficial microorganisms are vigorously multiplying and metabolizing, can material transformation and beneficial metabolites be continuously formed. Therefore, the types of beneficial microorganisms in microbial fertilizers and whether their life activities are vigorous are the basis of their effectiveness, unlike other fertilizers that are ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 赵振元罗明树王明云
Owner THE IT ELECTRONICS ELEVENTH DESIGN & RES INST SCI & TECHNOLOGICAL ENG
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