Artificial reproduction method for Pseudobagras ussuriensis
A technology of artificial reproduction and Ussuri, which is applied in fish farming, application, climate change adaptation, etc. It can solve the problems of germplasm resource destruction and inconformity with the development direction of sustainable sea bream, and achieve high sperm maturity and fertilization rate Guaranteed effect
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[0049] The preparation method of the artificial compound feed containing testosterone hormone is: mix a small amount of flour in warm boiled water to form a thin paste, then evenly mix 17α-methyltestosterone (white powder, odorless, tasteless) into the thin paste, and then Fully mix this thin paste with artificial compound pellet feed to obtain artificial compound feed containing testosterone hormone, and finally put it in a cool and ventilated place to dry and then feed it.
[0050] Broodstock rearing ponds must be fed with artificial compound pellets with a protein content greater than 20%. Set up a bait platform in the parent fish pond, and position and feed on the bait platform. At 9:00 am and 17:00 pm, 30% and 70% were fed respectively. It is advisable to feed the amount of bait in 60 minutes each time. Pay attention to observe the activity of the fish when feeding the bait, and do not feed the bait excessively. See Table 1 for the daily feeding amount. When the water ...
Embodiment 1
[0073] The inventor did the first experiment according to the above-mentioned technical scheme from February to June, 2010, and its specific implementation method is as follows:
[0074] (1) Selection of broodstock
[0075] In February, 660 male broodstock (average 215.3 g per fish) were selected from 3-year-old and above Ussuri broodstock with large individuals, strong physique, no disease, complete fin rays, and no damage.
[0076] (2) Breeding of broodstock
[0077] The female and male broodstock were put into different breeding ponds for cultivation. From April 22 (when the pond water temperature reached 17°C), 17α-methyltestosterone (17α-MT) was added to the breeding ponds of the male broodstock. The artificial compound feed was fed continuously for 30-36 days (1 and 2 batches of artificial breeding were continuously fed for 30 days, and artificial breeding batches 3 and 4 were continuously fed for 36 days, Table 3). The artificial compound feed containing 17α-methyltes...
Embodiment 2
[0085] The inventor has done the second experiment according to the above-mentioned technical scheme from February to June, 2011, and its specific implementation method is as follows:
[0086] (1) Selection of broodstock
[0087] In February, large, healthy, disease-free, complete fin rays and no damage were selected from 3-year-old and above Ussuri broodstock. Among them, 854 male broodstock (average 206.5 g per fish).
[0088] (2) Breeding of broodstock
[0089] The female and male broodstock were put into different breeding ponds for cultivation. From April 22 (when the pond water temperature reached 17°C), 17α-methyltestosterone (17α-MT) was added to the breeding ponds of the male broodstock. Artificial compound feed, continuous feeding for 28-38 days (artificial breeding 1, 2 batches of continuous feeding for 28 days, artificial breeding 3, 4 batches of continuous feeding for 32 days, artificial breeding 5, 6, 7 batches of continuous feeding 38 days. Table 4). The arti...
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