Method for regulating activity of soil matrix enzyme with Bacillus thuringiensis fermentation liquid

A technology of Bacillus thuringiensis and Bacillus aureus, which can be used in microorganism-based methods, soil lifting machinery, botanical equipment and methods, etc., and can solve problems such as lack of

Inactive Publication Date: 2012-07-25
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The environmental problems that may occur in the application of composting have also attracted people's attention, but there is still a lack of detailed and in-depth research on how to solve these problems. It is a

Method used

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  • Method for regulating activity of soil matrix enzyme with Bacillus thuringiensis fermentation liquid
  • Method for regulating activity of soil matrix enzyme with Bacillus thuringiensis fermentation liquid
  • Method for regulating activity of soil matrix enzyme with Bacillus thuringiensis fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] (1) Isolation of Bacillus thuringiensis:

[0097] 1) Weigh 10g of a fresh compost sample, put it into a 90ml sterile water triangular flask with 20 glass beads, shake it, so that the microorganisms in the sample are fully and evenly distributed in the liquid, and use a pipette gun in the triangular flask Take 1ml of mother liquor, add it into a large test tube filled with 9ml sterile water and mix well, this is l0 -1 Concentration of the diluted solution, according to this method were diluted to l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 Several concentrations of compost dilutions; use a pipette to draw 0.1 ml of concentration l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 The dilution solution was injected into the solid medium of beef extract peptone plate, and each dilution was repeated three times, and sterile water was used as the blank control; the bacterial suspension was evenly spread on the solid medium of whole beef extract peptone with a sterile glass spatula, s...

Embodiment 2

[0108] 1) Preparation of Bacillus thuringiensis fermentation broth:

[0109] A. Purify and cultivate the purchased Bacillus thuringiensis for 2 generations; then insert it into a 250 mL Erlenmeyer flask containing 50 mL of beef extract peptone liquid medium, cultivate at 180 r / min at 37°C, and use 600 nm wavelength for turbidimetric measurement to determine The OD value of the bacterial suspension is the ordinate, and the culture time is the abscissa, and the growth curve of the microorganism is drawn, and the time from the growth of Bacillus thuringiensis to the stationary phase is obtained according to the growth curve; the second-generation culture of the purified culture refers to: Pick up the colonies to be purified directly, and inoculate them into the solid medium of Gaoshi No. 1 plate prepared in advance, gently streak the plate from left to right, and place the culture plate upside down in a constant temperature incubator at 37°C for 1 Days, this process is the firs...

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Abstract

The invention discloses a method for regulating activity of a soil matrix enzyme with Bacillus thuringiensis fermentation liquid, which comprises the following steps: adding 30g of lawn soil into 6.5cm culture dishes, respectively adding 150mul compost microbial fermentation filtrates of different concentrations into the soil, adding an aseptic liquid culture medium into a control group, uniformly spreading 50 full Festuca arundinacea seeds in each culture dish, planting at room temperature, and measuring indicators after seed germination. Experimental results show that after the Bacillus thuringiensis fermentation liquid is added into the lawn soil matrix, the activity of the soil matrix enzyme can be significantly improved, meanwhile, the stress resistance of lawn plants is enhanced, and the growth of lawn plants is promoted.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the application of beneficial bacteria extracted from composting municipal domestic garbage. More specifically, it is a method for regulating the activity of soil matrix enzymes by adopting Bacillus thuringiensis fermented liquid. Background technique [0002] Composting refers to the process of degrading organic waste under the action of microorganisms (mainly bacteria) and transforming organic matter into stable humus under controlled conditions. The product after composting is called compost. The composting process can effectively reduce the volume of the mixture by 40%-50%, and can rely on the heat generated by metabolism in the high temperature stage to kill the pathogenic substances in the garbage. Composting is not a new technology, but it is in the interest of the environment to treat MSW in this way, because the composting process removes or reduces the ...

Claims

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Application Information

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IPC IPC(8): A01B77/00A01G1/00C12N1/20C12R1/07
Inventor 多立安赵树兰程田
Owner TIANJIN NORMAL UNIVERSITY
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