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Method for constructing half-sib family of Megalobrama Terminalis

A technique of half-sibs of Tuantou bream, applied in the field of construction of half-sib families of Tuantou bream, can solve the problems such as the construction method of half-sib families of Tuantou bream, which is easy to standardize the cultivation of families, solve the problem of asynchronous construction, Equipment requirements are not high

Inactive Publication Date: 2013-11-27
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] So far, there have been no relevant reports on the method of constructing half-sib families of bream

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  • Method for constructing half-sib family of Megalobrama Terminalis

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Embodiment Construction

[0024] The implementation and application of the present invention will be described below in conjunction with the embodiments.

[0025] The embodiment of the present invention is carried out in the national-level bream original species field (Ezhou City, Hubei Province), and the selected bream broodstock comes from the wild of Poyang Lake in Jiangxi Province, Liangzi Lake in Hubei Province and the silt lake in Hubei Province. There are 73 bream in total, including 24 females and 49 males.

[0026] The experiment started around December of that year, and the parents used to construct the half-sib family of the bream were raised separately, that is, the stocking density was 200kg / 666.7m 2 Breeding water. In parts by weight, the ration ratio of feeding is 30% grass carp polyculture floating commercial feed (purchased from Wuhan Gaolong Feed Co., Ltd.) and 70% green grass (such as planted ryegrass and Sudan grass) for fattening, It lasts until the pre-breeding in June of the se...

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Abstract

The invention belongs to the technical field of fresh water fish breeding, and specially relates to a method for constructing half-sib family of Megalobrama Terminalis. The method comprises (1) selecting Megalobrama Terminalis with body weight larger than 0.5 Kg, labeling with electronic label, and performing individual culture in advance for 6 months; (2) in June of current year, regulating sex gland development to reach synchronism, and performing primary injection of human chorionic gonadotropin (HCG) on female fish; (3) performing secondary injection of HCG and domperidone (DOM) on female and male parents 12 hr after the primary injection; (4) constructing half-sib family of Megalobrama Terminalis: one female parent crossed with two male parents for maternal half-sib family; one male parent crossed with two female parents for patrilineal half-sib family; (5) after the secondary injection, if ova automatically flow out when slightly pressing abdomen of Megalobrama Terminalis with finger, performing artificial insemination; (6) dividing collected ova into two equal parts, placing in stainless steel bowls, inseminating with sperm of two male fishes, debonding with loess water, rinsing with clear water, and placing in hatching jar.

Description

technical field [0001] The invention belongs to the field of fish breeding methods, and in particular relates to a method for constructing a half-sib family of bream. Background technique [0002] Giant bream (Megalobrama amblycephala Yih, 1955), also known as Wuchang fish, belongs to Osteichthyes, Cypriniformes, Cyprinidae, and genus Bream. Its natural distribution is limited to a few lakes in the middle and lower reaches of the Yangtze River. Bory bream is one of the important herbivorous economic fishes in my country. Due to its wide feeding habits, low cost, fast growth, high survival rate, and easy fishing, it can lay eggs and reproduce in ponds. It has the advantages of high rate, good shape, and moderate size, so it is widely promoted as an excellent herbivorous fish species throughout the country. [0003] In recent years, grouphead bream has become a best-selling species in the market, and the economic benefits of breeding are better. Therefore, there is an urgent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 曾聪王卫民高泽霞曹小娟罗伟
Owner HUAZHONG AGRI UNIV
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