Retinol binding protein assay kit based on latex particle coating

A detection kit and protein-binding technology, which is applied in the field of medical immunology in vitro diagnosis, can solve problems such as being susceptible to hemolysis interference and poor stability when opening bottles, and achieve high clinical application value

Active Publication Date: 2012-08-01
AILEX TECH GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It does not use detection signal method technology, and its minimum detection limit can only reach 10mg / l
At the same time, there are problems such as poor stability of opening the bottle and being easily interfered by hemolysis.
Clinically, it can only be used as an auxiliary diagnosis of renal function

Method used

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  • Retinol binding protein assay kit based on latex particle coating
  • Retinol binding protein assay kit based on latex particle coating
  • Retinol binding protein assay kit based on latex particle coating

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Embodiment 1

[0025] Reagent R1:

[0026]

[0027] The above-mentioned various components are added sequentially at room temperature, or added at the same time, or are packaged separately and prepared immediately before detection.

[0028] Reagent R2, including goat anti-human retinol binding protein polyclonal antibody coated polystyrene latex particle sensitization particles; phosphate buffer solution 35-60mmol / l, pH value 7.2-7.6; its preparation method is as follows:

[0029] Take the polystyrene latex particle that diameter is 45nm, after concentration is 45mmol / l, the Tris-Hcl buffer solution that pH value is 7.4 washes, and goat anti-human retinol binding protein polyclonal antibody with the ratio of 5:10 The mass ratio was mixed, and after mixing the two, they were adsorbed at 37°C for 8 hours, and then dialyzed to remove unlinked antibodies.

[0030] Add blocking solution and block for 2 hours. The main components of blocking solution are 0.1% skimmed milk powder and 0.2mm glyc...

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Abstract

The invention relates to the field of medical immonological in vitro diagnosis and in particular relates to a retinol binding protein assay kit based on latex particle coating. The assay kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 is a Tris-HCl buffer system, and comprises a Tris-HCl buffer solution, a polymer and ethylene diamine tetraacetic acid; the reagent R2 comprises goat anti human retinol binding protein polyclonal antibody coated polystyrene latex particle sensitized granules and a phosphate buffer solution; and the reference calibrator comprises bovine serum matrix, as well as 0.2-2.2% of antiseptic and 1-10% of stabilizer according to the volume percentage of the bovine serum matrix. Compared with the prior art, the assay kit can be used for assistant diagnosis of kidney diseases and assistant diagnosis of mal-nutrition, and has high clinical application value.

Description

[technical field] [0001] The invention relates to the field of in vitro medical immunodiagnosis, in particular to a latex particle-coated retinol-binding protein detection kit. [Background technique] [0002] Retinol-binding protein belongs to the Lipocalin superfamily, with a relative molecular mass of about 21×10 3 , the ligand is mainly all-trans retinol, ie vitamin A, Kd=20Nm, binding site 1. RBP is mainly responsible for binding and transporting vitamin A in plasma. After exogenous vitamin A enters the blood circulation system, it first combines with RBP, and the retinol-RBP complex further forms a ternary complex with transthyretin (TTR) and is transported. [0003] The liver is the main place for vitamin A storage and RBP synthesis and secretion. The secretion of RBP is strictly affected by the content of retinol in the body. Studies on RBP mutants and gene knockout mice have found that the absence of RBP in the blood has no significant effect on other aspects of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 李子樵房君江
Owner AILEX TECH GRP CO LTD
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