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Method for extracting total alkaloids from thalictrum plants

A technology of total alkaloids and pine grass, applied in the field of natural medicinal chemistry, can solve the problems of large amount of organic solvents, high production costs, environmental pollution, etc., and achieve the effect of low energy consumption and simple operation

Inactive Publication Date: 2012-08-08
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing methods for extracting total alkaloids from the plants of the genus Echinacea have many industrial "three wastes", serious environmental pollution, large amounts of organic solvents, high production costs, and cumbersome technical operations.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Pinus multifoliate is crushed into granules, 5kg is taken, extracted by percolation with 20 times the amount of 0.5% hydrochloric acid aqueous solution, the extract is filtered and added to a hollow cellulose ultrafiltration membrane with a molecular weight cut off of 3000, and the permeate is added with a cellulose ultrafiltration membrane with a molecular weight cut off of 100 The hollow cellulose nanofiltration membrane is concentrated, the concentrated solution is passed through the AB-8 macroporous resin column, and an appropriate amount of water is eluted, the column liquid and the washing liquid are collected to adjust the pH9 with ammonia water, and the precipitate is placed, centrifuged, and the precipitate is refluxed with 90% methanol to dissolve. Add 1% of the solution with activated carbon for decolorization, filter out the activated carbon from the decolorized solution, refrigerate and crystallize, and then recrystallize the crystals with 70% ethanol solutio...

Embodiment 2

[0016] Arrowhead Tang pine grass is crushed into granules, 10kg is taken, extracted by percolation with 15 times the amount of 1% hydrochloric acid aqueous solution, the extract is filtered and added to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 6000, and the permeate is added to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 300. Concentrate with cellulose nanofiltration membrane, pass the concentrated solution through D101 macroporous resin column, elute with appropriate amount of water, collect the lower column liquid and washing liquid, adjust the pH10 with ammonia water, place the precipitate, centrifuge, dissolve the precipitate with 99% methanol reflux, add the amount of solution Decolorize with 0.5% activated carbon, filter out the activated carbon from the decolorized solution, refrigerate and crystallize, and then recrystallize the crystals with 70% ethanol solution, dry to obtain the total alkaloids, ...

Embodiment 3

[0018] Crude tang pine grass into granules, take 20kg, use 15 times the amount of 2% hydrochloric acid aqueous solution to percolate and extract, filter the extract and add a hollow cellulose ultrafiltration membrane with a molecular weight cut off of 2000 for ultrafiltration, and then add cellulose with a molecular weight cut off of 200 to the permeate. The hollow cellulose nanofiltration membrane is concentrated, the concentrated solution is passed through the NK-9 macroporous resin column, and an appropriate amount of water is eluted, the column liquid and the washing liquid are collected to adjust the pH10 with ammonia water, and the precipitate is placed, centrifuged, and the precipitate is refluxed and dissolved with 95% methanol. Add 1% of the solution with activated carbon for decolorization, filter out the activated carbon from the decolorized solution, refrigerate and crystallize, and recrystallize the crystals with 95% ethanol solution, dry to obtain the total alkaloi...

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PUM

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Abstract

The invention discloses a method for extracting total alkaloids from thalictrum plants. The method includes the following steps of: crushing thalictrum plants, carrying out percolation extraction by adding 0.5-5% hydrochloric acid solution, utilizing an ultrafiltration membrane to carry out ultrafiltration on the filtered and extracted liquid, utilizing a nanofiltration membrane to carry out concentration on the filtered liquid, making the concentrated liquid pass through a macroporous resin column, carrying out elution with a proper amount of water, utilizing ammonia water to regulate the collected liquid dropping down from the macroporous resin column and water eluent to a ph from 8 to 10, allowing the obtained liquid to stand for precipitation, carrying out centrifugation, dissolving precipitate by methanol reflux, adding activated carbon for decolorization, carrying out cold crystallization, and carrying out recrystallization on crystallized substance with an ethanol solution to obtain a product. The method provided in the invention has the advantages of simple operation, little energy consumption in production, little pollution and easiness in industrialized production.

Description

technical field [0001] The invention belongs to the chemistry of natural medicines, in particular to a method for extracting total alkaloids from plants of the genus Tangsong. Background technique [0002] The genus Tangsong belongs to the family Ranunculaceae, with about 150 species. Distributed in the northern temperate zone, there are 67 species in my country, which are distributed all over the country. It is a natural anti-cancer plant resource and contains a large amount of alkaloids, such as tangconine, arrowhead tangconine, crane's tangconine, fenshi tangconine, Lesser tangconine, magnolialine, yellow tangconine, crane's tangconine, etc. Pharmacological studies have found that these alkaloids have anti-inflammatory, analgesic, bactericidal and anti-tumor effects. [0003] The method for extracting alkaloids from the plants of the genus Tang Echinacea is generally extracted by methanol or ethanol, extracted by organic reagents such as chloroform and benzene, or purifi...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P29/00A61P31/04A61P35/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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