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Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons

A technology of herbicide resistance gene and cotton resistance, which is applied in the field of identification of hybrid strains of transgenic cotton with bivalent insect resistance and herbicide resistance gene, and achieves the effects of fast speed, accurate identification, and simple preparation of elimination liquid

Inactive Publication Date: 2012-09-19
江西省棉花研究所
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AI Technical Summary

Problems solved by technology

Transgenic bivalent insect-resistant and herbicide-resistant gene (Cry1Ac+EPSPS) cotton is a new bivalent genetically modified insect-resistant and glyphosate-resistant cotton that came out in 2010, but currently this new bivalent insect-resistant and herbicide-resistant Glyphosate herbicide gene cotton still lacks supporting and special resistance identification miscellaneous plant elimination agent and method

Method used

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Embodiment Construction

[0015] A kind of method for identification of heterogeneous strains of transgenic cotton resistant to insects and herbicides with bivalent resistance, is to use 41% glyphosate isopropylamine salt 3.3ml / L+ 500,000 units of kanamycin 4000mg / L to eliminate the liquid The sprayer is sprayed on the cotton seedlings at the two-leaf and one-heart stage of the transgenic bivalent insect-resistant and glyphosate-resistant gene (Cry1Ac+EPSPS).

[0016] The elimination liquid is made of the following raw materials by weight percentage: glyphosate isopropylamine salt 0.1353%, kanamycin 4%, and the rest of water. The preparation method is to mix and dissolve the two agents in water to make the resistance identification complex In the strain removal solution, the content of glyphosate isopropylamine salt is 0.1353g / L, and the drug content of 500,000 units of kanamycin is 4000mg / L.

[0017] 10 days after spraying, record the level of phytotoxicity plant by plant, and the grading standards fo...

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Abstract

The invention provides a method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons. The method comprises the following steps: spraying a rejecting liquor, which is formed by 41% glyphosate isopropamide of 3.3 ml / L and 500 thousand unit kanamycin of 4000 mg / L, on insect-resistant and herbicide-resistant transgenic double-gene cotton seedlings at the two-leaf and one-bud stage by using a sprayer; and recording spray burn grades of the cotton seedlings one to one when 10 days pass away after spraying the rejecting liquor, wherein a high resistance grade is as follows: the cotton seedlings, 95-100% seed leaves and main leaves of which are not discolored and grow in a normal state, belong to a variety without resistance degradation; a resistance grade is as follows: the cotton seedlings, 90% seed leaves and main leaves of which turn yellow, belong to a variety with degraded resistance; a micro resistance grade is as follows: the cotton seedlings, 80% seed leaves and main leaves of which turn yellow and are locally withered due to water loss, belong to a variety with seriously degraded resistance; and a resistance-free grade is as follows: the cotton seedlings, 70% seed leaves of which are withered and main leaves and interior leaves of which are blackened and rotten, belong to a variety without resistance; and the method for rejecting the abnormal plants through resistance identification of the insect-resistant and herbicide-resistant transgenic double-gene cottons, provided by the invention, has the advantages of accuracy for identification, high speed and simplicity for preparing the rejecting liquor.

Description

technical field [0001] The invention relates to a method for identifying hybrid plants of cotton transfected with bivalent insect-resistant and herbicide-resistant genes (Cry1Ac+EPSPS). Background technique [0002] During the planting process of cotton varieties, due to artificial and natural factors (birds, insects) and other factors, the varieties will be adulterated and degraded, thereby affecting the yield. The traditional improvement method is to use cultural methods to purify the seeds of rejuvenated varieties. In the mid-1990s, after the advent of Bt-genetic insect-resistant cotton, it faced the problems of variety resistance degradation and seed hybridization. For this reason, researchers from the Cotton Research Institute of the Chinese Academy of Agricultural Sciences applied kanamycin 4000mg / l dilution in seedling cultivation. Spray when the cotton grows 1-2 true leaves, and then remove the non-transgenic cotton hybrids in the transgenic Bt gene insect-resistant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01H1/04
Inventor 张兴华李捷
Owner 江西省棉花研究所
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