Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons

Abstract

The invention provides a method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons. The method comprises the following steps: spraying a rejecting liquor, which is formed by 41% glyphosate isopropamide of 3.3 ml/L and 500 thousand unit kanamycin of 4000 mg/L, on insect-resistant and herbicide-resistant transgenic double-gene cotton seedlings at the two-leaf and one-bud stage by using a sprayer; and recording spray burn grades of the cotton seedlings one to one when 10 days pass away after spraying the rejecting liquor, wherein a high resistance grade is as follows: the cotton seedlings, 95-100% seed leaves and main leaves of which are not discolored and grow in a normal state, belong to a variety without resistance degradation; a resistance grade is as follows: the cotton seedlings, 90% seed leaves and main leaves of which turn yellow, belong to a variety with degraded resistance; a micro resistance grade is as follows: the cotton seedlings, 80% seed leaves and main leaves of which turn yellow and are locally withered due to water loss, belong to a variety with seriously degraded resistance; and a resistance-free grade is as follows: the cotton seedlings, 70% seed leaves of which are withered and main leaves and interior leaves of which are blackened and rotten, belong to a variety without resistance; and the method for rejecting the abnormal plants through resistance identification of the insect-resistant and herbicide-resistant transgenic double-gene cottons, provided by the invention, has the advantages of accuracy for identification, high speed and simplicity for preparing the rejecting liquor.

Description

technical field [0001] The invention relates to a method for identifying hybrid plants of cotton transfected with bivalent insect-resistant and herbicide-resistant genes (Cry1Ac+EPSPS). Background technique [0002] During the planting process of cotton varieties, due to artificial and natural factors (birds, insects) and other factors, the varieties will be adulterated and degraded, thereby affecting the yield. The traditional improvement method is to use cultural methods to purify the seeds of rejuvenated varieties. In the mid-1990s, after the advent of Bt-genetic insect-resistant cotton, it faced the problems of variety resistance degradation and seed hybridization. For this reason, researchers from the Cotton Research Institute of the Chinese Academy of Agricultural Sciences applied kanamycin 4000mg / l dilution in seedling cultivation. Spray when the cotton grows 1-2 true leaves, and then remove the non-transgenic cotton hybrids in the transgenic Bt gene insect-resistant ...

AI Technical Summary

Problems solved by technology

Transgenic bivalent insect-resistant and herbicide-resistant gene (Cry1Ac+EPSPS) cotton is a new bivalent genetically modified insect-resistant and glyphosate-resistant cotton that came out in 2010, but currently this new bivalent insect-resistant and herbicide-resistant Glyphosate herbicide gene cotton still lacks supporting and special resistance identification miscellaneous plant elimination agent and method