Quaternized chitosan/siRNA composite particle-loaded skin regeneration material and preparation method thereof
A technology of quaternized chitosan and composite particles, which is applied in the field of skin regeneration materials and its preparation, can solve the problems of inability to rebuild skin structure and function, skin ulceration and itching, skin tissue deformity and dysfunction, etc. The effect of synthesis, inhibition of scarring, and improvement of degradation properties
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Embodiment 1
[0028] A quaternized chitosan solution with a concentration of 2 mg / mL and a siRNA (Smad2-siRNA) solution that inhibits Smad2 protein (Smad2-siRNA) with a concentration of 0.2 mg / mL were prepared with ultrapure water; the quaternized chitosan solution and Smad2- The siRNA solution was mixed in equal volumes, vortexed and oscillated evenly, and then left to stand to prepare quaternized chitosan / Smad2-siRNA composite particles in a suspended and dispersed state. The morphology of the composite particles was observed by transmission electron microscopy as follows: figure 1 shown. Be that the acetic acid solution that mass concentration is 1% prepares the collagen solution that mass concentration is 0.7% and the chitosan solution that mass concentration is 0.7% respectively; Chitosan solution is dripped in the collagen solution so that the mass ratio of chitosan and collagen 5:5, stir evenly and vacuum degassing to obtain the collagen-chitosan mixed solution; inject the collagen-c...
Embodiment 2
[0030] Prepare a quaternized chitosan solution with a concentration of 5 mg / mL and a siRNA (Smad3-siRNA) solution that inhibits Smad3 protein at a concentration of 0.5 mg / mL with ultrapure water; the quaternized chitosan solution and Smad3- The siRNA solution was mixed in equal volumes, vortexed evenly, and then left to stand to prepare quaternized chitosan / Smad3-siRNA composite particles in a suspended and dispersed state. Be that the acetic acid solution that mass concentration is 3% is prepared respectively that mass concentration is the collagen solution of 0.5% and the chitosan solution that mass concentration is 0.5%; Chitosan solution is dripped in the collagen solution so that the mass ratio of chitosan and collagen 1:9, stir evenly and vacuum degassing to obtain the collagen-chitosan mixed solution; inject the collagen-chitosan mixed solution into the mold, at -50 oC Freeze and lyophilize at low temperature to obtain a collagen-chitosan three-dimensional porous scaff...
Embodiment 3
[0032] A quaternized chitosan solution with a concentration of 1 mg / mL and a solution for inhibiting transforming growth factor β1-siRNA (TGFβ1-siRNA) with a concentration of 0.1 mg / mL were prepared with ultrapure water; the quaternized chitosan solution and TGFβ1-siRNA solutions were mixed in equal volumes, vortexed and oscillated evenly, and then left to stand to obtain quaternized chitosan / TGFβ1-siRNA composite particles in a suspended and dispersed state; the mass concentration was prepared with 1% acetic acid solution with a mass concentration of 0.3 % collagen solution and a mass concentration of 0.3% chitosan solution; the chitosan solution is dropped into the collagen solution so that the mass ratio of chitosan and collagen is 3:7, stirred evenly and vacuum defoaming, to obtain collagen- Chitosan mixed solution; inject the collagen-chitosan mixed solution into the mold at -80 oC Freeze and lyophilize at low temperature to obtain a three-dimensional collagen-chitosan p...
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