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Quaternized chitosan/siRNA composite particle-loaded skin regeneration material and preparation method thereof

A technology of quaternized chitosan and composite particles, which is applied in the field of skin regeneration materials and its preparation, can solve the problems of inability to rebuild skin structure and function, skin ulceration and itching, skin tissue deformity and dysfunction, etc. The effect of synthesis, inhibition of scarring, and improvement of degradation properties

Inactive Publication Date: 2012-09-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although scar repair can restore the integrity of the wound, it cannot rebuild the original structure and function of the skin, which can easily cause skin tissue deformity and dysfunction, and can also lead to local skin ulceration and itching.

Method used

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  • Quaternized chitosan/siRNA composite particle-loaded skin regeneration material and preparation method thereof
  • Quaternized chitosan/siRNA composite particle-loaded skin regeneration material and preparation method thereof
  • Quaternized chitosan/siRNA composite particle-loaded skin regeneration material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A quaternized chitosan solution with a concentration of 2 mg / mL and a siRNA (Smad2-siRNA) solution that inhibits Smad2 protein (Smad2-siRNA) with a concentration of 0.2 mg / mL were prepared with ultrapure water; the quaternized chitosan solution and Smad2- The siRNA solution was mixed in equal volumes, vortexed and oscillated evenly, and then left to stand to prepare quaternized chitosan / Smad2-siRNA composite particles in a suspended and dispersed state. The morphology of the composite particles was observed by transmission electron microscopy as follows: figure 1 shown. Be that the acetic acid solution that mass concentration is 1% prepares the collagen solution that mass concentration is 0.7% and the chitosan solution that mass concentration is 0.7% respectively; Chitosan solution is dripped in the collagen solution so that the mass ratio of chitosan and collagen 5:5, stir evenly and vacuum degassing to obtain the collagen-chitosan mixed solution; inject the collagen-c...

Embodiment 2

[0030] Prepare a quaternized chitosan solution with a concentration of 5 mg / mL and a siRNA (Smad3-siRNA) solution that inhibits Smad3 protein at a concentration of 0.5 mg / mL with ultrapure water; the quaternized chitosan solution and Smad3- The siRNA solution was mixed in equal volumes, vortexed evenly, and then left to stand to prepare quaternized chitosan / Smad3-siRNA composite particles in a suspended and dispersed state. Be that the acetic acid solution that mass concentration is 3% is prepared respectively that mass concentration is the collagen solution of 0.5% and the chitosan solution that mass concentration is 0.5%; Chitosan solution is dripped in the collagen solution so that the mass ratio of chitosan and collagen 1:9, stir evenly and vacuum degassing to obtain the collagen-chitosan mixed solution; inject the collagen-chitosan mixed solution into the mold, at -50 oC Freeze and lyophilize at low temperature to obtain a collagen-chitosan three-dimensional porous scaff...

Embodiment 3

[0032] A quaternized chitosan solution with a concentration of 1 mg / mL and a solution for inhibiting transforming growth factor β1-siRNA (TGFβ1-siRNA) with a concentration of 0.1 mg / mL were prepared with ultrapure water; the quaternized chitosan solution and TGFβ1-siRNA solutions were mixed in equal volumes, vortexed and oscillated evenly, and then left to stand to obtain quaternized chitosan / TGFβ1-siRNA composite particles in a suspended and dispersed state; the mass concentration was prepared with 1% acetic acid solution with a mass concentration of 0.3 % collagen solution and a mass concentration of 0.3% chitosan solution; the chitosan solution is dropped into the collagen solution so that the mass ratio of chitosan and collagen is 3:7, stirred evenly and vacuum defoaming, to obtain collagen- Chitosan mixed solution; inject the collagen-chitosan mixed solution into the mold at -80 oC Freeze and lyophilize at low temperature to obtain a three-dimensional collagen-chitosan p...

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Abstract

The invention discloses a quaternized chitosan / siRNA composite particle-loaded skin regeneration material and a preparation method thereof. The preparation method comprises the following steps: selecting quaternized chitosan with high biocompatibility and transmission efficiency as a siRNA carrier material, and complexing the quaternized chitosan and siRNA capable of suppressing the expression of scarring correlation factors to obtain quaternized chitosan / siRNA composite particles; and loading the quaternized chitosan / siRNA composite particles on a collagen-chitosan / silicon rubber double-layer scaffold through physisorption to finally obtain the skin regeneration material, wherein the skin regeneration material can reduce the expression level of the scarring correlation factors in the skin repairing process through in-situ transfection of the composite particles on the skin defect wound surface, and suppresses excessive collagen deposition and scarring while effectively promoting regenerative repair of the defected skin. The skin regeneration material prepared by the method has excellent capacity of repairing wound surface and weakening scars, can be widely applied to repair and regeneration of whole-thickness skin defects, and has a good clinical application prospect.

Description

technical field [0001] The invention relates to a skin regeneration material loaded with gene active substances and a preparation method thereof, in particular to a collagen-chitosan / silicone rubber skin regeneration material loaded with quaternized chitosan / siRNA composite particles and a preparation method thereof . Background technique [0002] The skin is the largest organ in the human body, accounting for about 16% of the body weight. The skin can be divided into three parts from the surface to the inside: the epidermis, the dermis and the subcutaneous tissue; its structure is highly complex, including a variety of cells and biological macromolecules such as collagen, elastin, glycoprotein or proteoglycan, as well as amino acids, Glucose, vitamins, inorganic salts and other small molecules. Due to extensive exposure to the external environment in daily life, the skin is easily damaged by burns, mechanical trauma, and chronic diseases (such as diabetes), which seriousl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/20A61L27/18A61L27/60A61L27/54C08J9/36C08J9/28C08J3/24C08J5/12
Inventor 马列刘幸高长有
Owner ZHEJIANG UNIV
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