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Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits

A technology for mogroside and farnesol, which is applied in the fields of simultaneously extracting mogroside V and farnesol, and extracting mogroside V and farnesol, can solve problems such as farnesol not being well utilized, and achieves improved utilization. efficiency, saving resources and simplifying the production process

Active Publication Date: 2012-09-19
长沙卫一生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0040] In summary, the current utilization of Luo Han Guo is mainly used to extract mogroside V, which is used as a sweetener, while farnesol, which has insect juvenile hormone activity, has not been well utilized

Method used

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  • Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits
  • Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits

Examples

Experimental program
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Effect test

Embodiment 1

[0064] 100 kg of dried Luo Han Guo was used to break the shell and crush, add 1500L of 50% ethanol to mix, heat to 70°C for reflux extraction for about 4 hours, filter twice with three layers of gauze and filter once with ceramic membrane, after filtering to remove slag, at 70 Concentrate under reduced pressure at ℃ until 13.45kg of dry matter is obtained, add 55L of water to the dry matter to dissolve, filter through a ceramic membrane, dissolve the filter residue with 30L ethyl acetate, and perform chromatography on a silica gel column, using petroleum ether solution. Elute to obtain 120L of eluent II, put the filtrate on AB-8 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 60L of eluent I with 60% ethanol, and concentrate eluent I to 6 L, add 6 L of acetone solution, crystallize and recrystallize overnight at room temperature, and obtain 1.14 kg of pure mogroside V; concentrate the eluent II under reduced pressure to obtain...

Embodiment 2

[0066] 200 kg of dried Luo Han Guo was used to break the shell and crush, add 4500L of 50% ethanol to mix, heat to 70°C for reflux extraction for about 4 hours, filter twice with three layers of gauze and filter once with ceramic membrane, after filtering to remove slag, at 70 Concentrate under reduced pressure at ℃ until 28.68kg of dry matter is obtained, add 120L of water to the dry matter to dissolve, filter through a ceramic membrane, dissolve the filter residue with 50L of ethyl acetate, and perform chromatography on a silica gel column, using petroleum ether solution. Elute to obtain 280L of eluent II, put the filtrate on AB-8 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 150L of eluent I with 60% ethanol, and concentrate eluent I to 15L, add 30L acetone solution, crystallize and recrystallize overnight at room temperature, and obtain 2.26kg of pure mogroside V; concentrate the eluate II under reduced pressure to obtai...

Embodiment 3

[0068] 400 kg of dried Luo Han Guo was used to break the shell and pulverize, add 7000L of 50% ethanol to mix, heat to 70°C for reflux extraction for about 4 hours, filter twice with three layers of gauze and filter once with ceramic membrane, after filtering to remove slag, at 70 Concentrate under reduced pressure at ℃ until 55.5kg of dry matter is obtained, add 220L of water to the dry matter to dissolve, filter through a ceramic membrane, dissolve the filter residue with 80L of ethyl acetate, and perform chromatography on a silica gel column, using petroleum ether solution. Elute to obtain 200L of eluent II, put the filtrate on AB-8 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 150L of eluent I with 60% ethanol, and concentrate eluent I to 30L, add 30L acetone solution, crystallize and recrystallize overnight at room temperature, and obtain 4.25kg of pure mogroside V; concentrate the eluate II under reduced pressure to ob...

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Abstract

The invention discloses a method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits. Purified products of momordica grosvenori glycoside V and farnesol can be obtained by subjecting dry grosvenor momordica fruits to shelling, water extracting, filtering, resin adsorption, eluting by different-concentration ethanol, decoloring different eluent, crystallizing and recrystallizing. Reagents used in the method are all chemical reagents which are nontoxic, low-cost and massively produced, the mature normal reagent recovery technique can be used in the whole process, wastes discharged to the environment can be greatly reduced, operations are simple, production cost of the momordica grosvenori glycoside V and farnesol is greatly lowered, and the production process is simplified. Common raw materials and devices are used in the method, dependency on expensive raw materials and devices during industrial production is avoided, and accordingly production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting mogroside V and farnesol, and more specifically relates to a method for simultaneously extracting mogroside V and farnesol from the fruit of Luo Han Guo. Background technique [0002] Farnesol has rich biological activities: it has the activity of insect juvenile hormone, can be used as some insect sex pheromones, and is a natural insecticide against mites; it can be used to prevent and treat tumors; it can be used in perfumes, as Co-solvent, regulates the fluctuation of odor, emphasizes the smell of sweet floral perfume, and is especially suitable for lavender perfume; it can be used as a flavoring ingredient and is one of the 599 additives of cigarettes; this compound can be used in cosmetics as a deodorant, except for People who are allergic to perfume can be widely used; in addition, the compound can also inhibit the formation of mycelia of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00C07C33/02C07C29/76
Inventor 唐忠海
Owner 长沙卫一生物科技有限公司
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