Plate kit for identifying vibrio parahemolyticus toxigenic strain, and preparation and using methods for plate kit

A hemolytic Vibrio and kit technology, which is applied in biochemical equipment and methods, microorganism-based methods, and microbial determination/inspection, etc. Toxicity strain identification is cumbersome and other problems, to achieve the effect of improving identification methods, easy to observe and judge, easier to grasp and accept

Inactive Publication Date: 2012-10-03
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology

It mainly solves technical problems such as cumbersome identification of toxin-producing strains of Vibrio parah

Method used

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  • Plate kit for identifying vibrio parahemolyticus toxigenic strain, and preparation and using methods for plate kit

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Embodiment Construction

[0008] First prepare the kit: mix 3.0g of yeast extract, 10.0g of peptone, 80.0g of sodium chloride, 30.0g of disodium hydrogen phosphate, 10.0g of glucose, 15.0g of agar, and 1000.0ml of distilled water, and adjust the pH to 8.0 + 0.2; heat to 100°C, keep for 30 minutes, cool to 46°C-50°C, mix with 50ml fresh rabbit blood containing 0.3% sodium citrate by volume, and pour into the plate. Use after the surface of the tablet is dry. Since the activity of rabbit blood in the culture medium is about 28 days old, the plate should be used within 4 weeks.

[0009] The ready-to-use Vibrio parahaemolyticus toxin-producing strain identification plate usage method:

[0010] ⑴ Apply freshly cultivated pure bacterial lawn or single colony;

[0011] ⑵ On the identification plate of Vibrio parahaemolyticus toxin-producing strains, pick a small area (a circular area with a diameter of about 1-3 mm) of the tested bacteria with an inoculation loop, and incubate in a 36°C incubator for 18 hou...

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Abstract

The invention relates to a kit for identifying a vibrio parahemolyticus toxigenic strain by using the hemolysis of the vibrio parahemolyticus toxigenic strain, and preparation and using methods for the kit, and mainly solves the problems that the technical problems that the conventional methods for identifying the vibrio parahemolyticus toxigenic strain are complex and cannot be unified easily, and the vibrio parahemolyticus toxigenic strain cannot be used as the conventional detection target to be used. According to technical scheme, the ready-to-use vibrio parahemolyticus toxigenic strain identification plate kit is characterized by comprising a vibrio parahemolyticus toxigenic strain identification culture medium which comprises 3.0g of yeast extract, 10.0g of peptone, 80.0g of sodium chloride, 30.0g of disodium hydrogen phosphate, 10.0g of glucose, 15.0g of agar, 1000.0ml of distilled water, and 50ml of fresh rabbit blood; and the pH of the culture medium is adjusted to be 8.0+/-0.2; and the rabbit blood contains 0.3 volume percent of anticoagulant of sodium citrate. A blood plate is prepared by using the hemolysis characteristic of the vibrio parahemolyticus toxigenic strain, the hemolysis of vibrio parahemolyticus is observed by a one-step inoculation-culturing method, and the effects of quickly identifying the toxigenic strain quickly and easily can be achieved.

Description

technical field [0001] The invention relates to an identification method of toxin-producing strains of vibrio parahaemolyticus among food-borne pathogenic bacteria, namely a kit for identification by using the hemolysis phenomenon of toxin-producing strains of vibrio parahaemolyticus, a preparation method and a use method. Background technique [0002] At present, Vibrio parahaemolyticus is an important pathogen causing foodborne diseases at home and abroad, especially in coastal countries. The strain contains the virulence factor thermostable direct hemolysin (TDH), which is the main strain causing food poisoning incidents, so the isolation and identification of toxin-producing strains of Vibrio parahaemolyticus is particularly important. The current method for detecting toxin-producing strains of Vibrio parahaemolyticus mainly relies on molecular biology methods, that is, using polymerase chain amplification (PCR) to detect the heat-resistant direct hemolysin gene ( TT ),...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/63
Inventor 张红芝许学斌陈长怡
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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