Thermophilic facultative aerobic bacteria UTM601 and application thereof

A technology of anaerobic bacillus and strains, applied in the field of environmental microorganisms, can solve the problems of no public reports of thermophilic and facultative bacteria, and achieve the effects of avoiding ventilation management control problems, low cost, and simple operation

Active Publication Date: 2013-03-27
大地绿源环保科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no public report on the application of thermophilic facultative bacteria in high-temperature composting as an inoculum

Method used

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  • Thermophilic facultative aerobic bacteria UTM601 and application thereof
  • Thermophilic facultative aerobic bacteria UTM601 and application thereof
  • Thermophilic facultative aerobic bacteria UTM601 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of thermophilic facultative bacteria UTM601

[0032]Weigh 10g of high-temperature period sludge compost sample (collected from the compost fermentation workshop of Dadi Lvyuan Environmental Protection Technology (Beijing) Co., Ltd.) into a 250ml triangular flask filled with 10 glass beads and 90ml sterile water, and shake it on a shaker at 200rpm 30 minutes, then take it out and let it stand for 30 minutes, take 10ml supernatant and connect to 90ml NA culture solution (beef extract 4g, peptone 8g, sodium chloride 4g, agar 16g, plant gel 8g, distilled water 1000ml, pH7.2) Placed on a water bath shaker, at 60°C, variable speed acclimation culture: every 6 hours as a time unit, with 50rpm as a variable speed unit, gradually increase the speed until it reaches 150rpm, then gradually reduce the speed, which is One acclimatization cycle; after completing 3 acclimatization cycles, take 10ml of acclimatization liquid and transfer it to new...

Embodiment 2

[0038] The preparation of embodiment 2 compost inoculants (1)

[0039] (1) Preparation of activation solution: take 1 slant surface stored at 4°C of the microorganism UTM601 of the present invention and inoculate it into 5 test tubes containing 10ml of sterilized NA liquid culture solution, and cultivate it in a constant temperature water bath at 55°C for 1.5 days to obtain slant activation. bacteria liquid. The OD600 of the activated bacterial liquid on the inclined plane was 0.8.

[0040] (2) Preparation of seed solution: Inoculate one test tube of the above-mentioned slant-activated bacterial solution into 1L of sterile NA liquid culture solution, culture on a water-bath shaker with shaking at a temperature of 55°C, and an oscillation frequency of 50rpm, and cultivate for 3 days to obtain a seed solution. The OD600 of the seed solution was 1.2.

[0041] (3) Preparation of fermented seed liquid: the above seed liquid was inoculated into a sterilized fermenter at an inoculu...

Embodiment 3

[0043] The preparation of embodiment 3 compost inoculants (2)

[0044] (1) Prepare the activation solution, and the culture conditions are: take 1 slant stored at 4°C of the microorganism UTM601 of the present invention and inoculate it into 5 sterilized NA liquid culture medium test tubes containing 10ml, and cultivate it in a constant temperature water bath at 50°C for 2 days , the OD of the activated bacterial solution on the inclined plane 600 is 1.2.

[0045] (2) Prepare the seed liquid, cultivate the temperature at 50°C, shake the frequency at 160rpm, and obtain the seed liquid after culturing for 4 days, the OD of the seed liquid 600 is 1.5.

[0046] (3) In the preparation of the fermented seed liquid, the inoculation amount of the above seed liquid is 15% (v / v), and the conditions for the expanded fermentation culture are: temperature 50°C, ventilation for 20 minutes at intervals of 5 hours, volume ratio of ventilation volume 0.3:1 , stirred at 160rpm, and cultivate...

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Abstract

The invention provides a thermophilic facultative aerobic bacteria (anoxybacillus pushchinoensis) UTM601, whose preservation number is CGMCC No.5929. The bacterial strain UTM601 grows well under micro-aerobic and aerobic environments at 50-70 DEG C, and has the characteristics of being capable of well adapting to compost high-temperature environment with high temperature and inhomogenous oxygen content distribution and degrading organic substances quickly. The invention also provides the method of using the bacterial strain to prepare compost inoculum. The bacterial strain of the invention uses organic waste as carriers to prepare solid-state microorganism compost inoculum, which is low in cost, and the bacterial strain will not influence material moisture when the bacterial strain is applied to compost. With lower multiple horizontal ventilation volume, the solid-state microorganism compost inoculum adopted by the invention for high-temperature compost can realize the high-temperature fermentation of materials and avoid complex ventilation management and control problems during the composting process, and the method has the advantages of simple operation and energy saving.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a thermophilic facultative bacteria strain UTM601 and its application in high-temperature composting. Background technique [0002] Composting is a process in which organic matter is mineralized, humified and harmless through high-temperature fermentation under the action of microorganisms to become decomposed fertilizer. As a microbiological process, high-temperature composting depends on a certain amount and vitality of microbial sources. With the industrialization and commercialization of composting, microbial inoculant has become an important technology and has been applied. [0003] At present, the fermentation process used in high-temperature composting is generally aerobic fermentation, and the microbial inoculant is mainly mesophilic aerobic bacteria. Aerobic fermentation must maintain a certain oxygen concentration. However, composting is a comple...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/01
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 大地绿源环保科技(北京)有限公司
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