Primers used for rapid detection of Neofusicoccum parvum and application thereof

A technology for grape canker bacteria and strains, which is applied to a primer for rapid detection of grape canker bacteria and its application field, can solve problems such as no molecular detection technology, and achieve the effects of shortening identification time, high sensitivity, and overcoming cumbersome steps.

Inactive Publication Date: 2012-10-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

However, in terms of detection of grape canker, there is no s

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  • Primers used for rapid detection of Neofusicoccum parvum and application thereof
  • Primers used for rapid detection of Neofusicoccum parvum and application thereof
  • Primers used for rapid detection of Neofusicoccum parvum and application thereof

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[0022] Example 1. Primers used for rapid detection of neofusicoccum parvum and their applications

[0023] 1. Obtainment of primers for rapid detection of neofusicoccum parvum

[0024] The 5 ITS sequences of Neofusicoccum parvum sequenced by our laboratory and the ITS sequences of 20 different species of Botryococcus in GeneBank (accession numbers are: GU251147; HQ529768; JN088510; GU292676; FJ904819; EU683673; HQ288230; EF585518; JN088050; HQ625634; FJ888469; JN849098; JN035222; FJ904815; EU331085; DQ458889; HQ629954; AY236954; EU520175; AB454278) for homology comparison, and design specific primers for Neofusicoccum parvum based on the differences between Neofusicoccum parvum and other species , The resulting primer is the primer Bp-F / Bp-R of the present invention. The primer sequence is: upstream forward primer Bp-F sequence: 5'-AAAACTCCAGTCAGTGAACTTC-3' (sequence 1 in the sequence list); downstream reverse primer Bp-R sequence: 5'-GGTCAACCTTGAGAAATAATTCAAAG-3' (sequence 2 in t...

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Abstract

The invention discloses a pair of primers used for rapid detection of Neofusicoccum parvum strains and application thereof. Nucleotide sequences of the pair of the primers are respectively represented by sequence 1 and sequence 2 in a sequence table, and the primers can specifically amplify the product of 370bp in Neofusicoccum parvum and have a sensitivity of 10 pg to genomic DNAs of the Neofusicoccum parvum strain. It is proved that the pair of the primers can be used for rapid reliable detection and identification of the Neofusicoccum parvum strain in disease samples of grapes in the field, and the problems of cumbersome steps, a long identification period and the like in traditional identification methods are overcome.

Description

technical field [0001] The invention relates to a pair of rapid molecular detection primers for grape canker bacterium Neofusicoccum parvum strain and application thereof. Background technique [0002] Grape canker (Grape Botryosphaeriaceae Canker) is a disease caused by the fungus infection of Botryosphaeriaceae (Botryosphaeriaceae). 14 countries including South Africa and China have occurred and caused serious losses. At present, it is known that 15 species of Botryosphaeriaceae can cause the disease (Auger J, Esterio M, Ricke G, et al. Black dead arm and basal canker of Vitis vinifera cv. Red Globe caused by Botryosphaeria obtusa in Chile [J].Plant Disease.2004,88(11):1286.Niekerk J M,Fourie P H,Hallenn F,et al.Botryosphaeria spp.as grapevine trunk disease pathogens[J].Phytopathologia Mediterranea.2006,45(4): 43-54.U Rbez-Torres J R,Peduto F,Striegler R K,et al.Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri[J].Fungal...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68C12R1/645
Inventor 张玮李兴红燕继晔严红
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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