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Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously

A technology of flavonoids and white syrup, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time consumption, and achieve the effect of reducing steps and time consumption

Inactive Publication Date: 2014-07-30
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reported method is the application of high performance liquid chromatography for separate quantitative determination of hyperoside, quercitrin and quercetin, making the time-consuming determination of the content of various flavonoids in Manshan white syrup longer

Method used

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  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously
  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously
  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of standard solution: Weigh 20mg hyperoside, 10mg quercetin, and 10mg quercetin reference substance and mix them, and then dissolve the mixed reference substance with methanol and dilute the volume to obtain a total concentration of 4.000 mg / mL mother liquor, and then dilute the mother liquor into 6 concentration gradients, the concentration gradients were 0.01, 0.1, 0.2, 0.5, 0.8 and 1.0 times the concentration of the mother liquor; among them, hyperoside, quercetin, and quercetin The purity of the vegetarian reference substance is 98%.

[0026] 2. Preparation of the test solution: Take 18mL syrup and add 6mL water-saturated n-butanol for extraction (that is, the volume ratio of syrup and water-saturated n-butanol is 3:1), extract 3 times, combine the extracts, and The combined extracts were concentrated under reduced pressure to dryness, and then dissolved in methanol and transferred to a 25mL volumetric flask for constant volume (and then filtered with a ...

Embodiment 2

[0040] Preparation of the test solution: Take 18mL of syrup and add 18mL of water-saturated n-butanol for extraction (that is, the volume ratio of syrup and water-saturated n-butanol is 1:1), extract 5 times in total, combine the extracts, and combine them The extract was concentrated to dryness under reduced pressure, then dissolved by adding methanol and transferred to a 25mL volumetric flask, and then filtered with a 45nm pore filter membrane to obtain the extract of Manshanbai syrup flavonoids, which is the test solution ;

[0041] Determination: Take the test solution prepared in this embodiment and inject it into a high performance liquid chromatograph consistent with the chromatographic conditions of Example 1 for analysis and determination, to obtain a high performance liquid chromatogram of the test solution, specifically, the high efficiency of the test solution Liquid chromatogram such as image 3 As shown, and the peak area is obtained through integration by the softw...

Embodiment 3

[0043] Preparation of the test solution: Take 18mL of syrup and add 36mL of water-saturated n-butanol for extraction (that is, the volume ratio of syrup and water-saturated n-butanol is 1:2), extract once, and concentrate the extract under reduced pressure to dryness. Then add methanol to dissolve it and transfer it to a 25mL volumetric flask to make the volume constant, then filter with a 45nm pore size filter to obtain the extract of Manshanbai syrup flavonoids, which is the test solution;

[0044] Determination: Take the test solution prepared in this embodiment and inject it into a high performance liquid chromatograph consistent with the chromatographic conditions of Example 1 for analysis and determination, to obtain a high performance liquid chromatogram of the test solution, specifically, the high efficiency of the test solution Liquid chromatogram such as Figure 4 As shown, and the peak area is obtained through integration by the software of the chromatographic instrume...

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Abstract

The invention provides a method for measuring the contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously. The method comprises the following steps of: firstly preparing standard solution and liquid to be measured, then carrying out high-performance liquid chromatography detection on the standard solution and the liquid to be measured, wherein the chromatographic condition is as follows: the chromatographic column is a C18 chromatographic column; the mobile phase is acetonitrile and water; the column temperature is 25 DEG C; the detection wavelength is 360nm; the sample size is 20muL; the change process of the mobile phase is 0-25min; the volume ratio of the acetonitrile and the water is 70:30; in 25-26min, the volume ratio of the acetonitrile and the water is changed from 70:30 isocratic gradient to 60:40; and in 26-55min, the volume ratio of the acetonitrile and the water is 60:40. The method has the advantages that the contents of the three flavonoid compounds in the rhododendron seniavinii syrup can be measured simultaneously, so that the measurement steps are reduced and the time consumed by measurement is reduced.

Description

【Technical Field】 [0001] The invention relates to a method for simultaneous determination of multiple components, in particular to a method for simultaneous determination of the content of three flavonoids in Manshanbai syrup. 【Background technique】 [0002] Manshanbai is the dry twigs and leaves of Rhododendron senniavinii Maxim., a local medicinal material of Fujian Province, which has the effect of expectorating and relieving cough; Manshanbai cough syrup series products are mainly Manshanbai medicine The clinical application of raw materials is Chinese patent medicine. The flavonoids such as hyperoside, quercetin and quercetin in Manshanbai cough syrup series products are the material basis for its pharmacological activity, and the content levels of hyperoside, quercetin and quercetin It directly affects the quality of medicinal materials and ultimately the quality of finished medicines. The development and application of a rapid quantitative determination method for flavon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 黄颖桢陈菁瑛赵云青林峰
Owner 福建省农业科学院农业生物资源研究所
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