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Rapid propagation method of tissue culture for zelkova schneideriana hand.-Mazz

A tissue culture and beech tree technology, applied in the field of plant reproduction, can solve the problems of low reproduction coefficient and poor consistency, and achieve the effect of improving reproduction coefficient

Inactive Publication Date: 2012-11-28
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The consistency of clustered bud seedlings obtained by tissue culture of terminal buds is relatively poor, and the reproduction coefficient is low (27~125); the regenerated plants produced by tissue culture of leaf-induced callus have poor consistency with the mother plant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Disinfect the young shoots of beech trees with buds in spring that were selected in the current year with 75% alcohol for 30-35 seconds, rinse with sterile water for 3 times, disinfect with 0.1% mercury liter for 8 minutes, and rinse with sterile water for 3 times ;

[0024] (2) Cutting the branches treated in step (1) into 1 cm long stem segments containing at least one axillary bud, implanting them in a bud induction medium and culturing them for 28 to 30 days to obtain clustered buds; wherein, the bud induction medium is : WPM + 3.0 mg.L -1 BA, agar 6.5 g.L -1 , sucrose 20 g.L -1 , the pH value is 5.7, and the medium is autoclaved for 20 minutes; the induction rate of clustered buds is 84-90%;

[0025] (3) transfer the clustered buds grown after the induction culture in step (2) to the subculture medium and culture them for 60 days to proliferate the buds, and the bud proliferation coefficient is 8-10; wherein, the subculture medium is : WPM+3.0 mg.L -1 B...

Embodiment 2

[0031] Place the robust branches of the beech tree with axillary buds that have fallen leaves in winter in a beaker filled with distilled water, and carry out hydroponics at 25°C for 15 to 30 days to obtain young shoots that have germinated from the axillary buds, and then perform the following steps;

[0032] (1) Disinfect young beech branches with 75% alcohol for 25-30 seconds, rinse with sterile water for 3 times, disinfect with 0.1% mercury liter for 12 minutes, and rinse with sterile water for 3 times;

[0033] (2) cutting the branches treated in step (1) into a 1.2cm-long stem segment containing at least one axillary bud, implanting them in a cluster bud induction medium and culturing them for 30 to 32 days to obtain cluster buds; wherein, the cluster bud induction Medium: WPM + 1.0 mg.L -1 BA+1.0 mg.L -1 NAA, agar 6.5 g.L -1 , sucrose 20 g.L -1 , the pH value is 5.7, and the medium is autoclaved for 20 minutes;

[0034] (3) transfer the clustered buds grown after ...

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Abstract

The invention discloses a rapid propagation method of tissue culture for zelkova schneideriana hand.-Mazz. According to the method, selecting stem segments with buds as explants, and appropriate culture mediums for each stage for the tissue culture, a large number of nursery stocks, which maintain the qualified characteristics of a mother stock, can be obtained; and the genotype and all the qualified characteristics of an original plant can be 100% maintained, thereby greatly improving the propagation coefficient.

Description

Technical field [0001] The present invention is a plant reproduction technology field, and it involves a fast way to make a cricket tree. Background technique [0002] Beech Zelkova Schneideriana Hand.-mazz.It is a precious and hard broad -leaved material tree species. The wood materials of the trees are hard and flexible. The texture is beautiful and shiny, and there is no special odor. It has become the first choice for many families to make indoor decoration and manufacturing high -end furniture, which is loved by consumers.At the same time, the trees of the trees are beautiful, and the leaves will show different colors with the season, with high gardening and ornamental value.Moreover, the pests and insect pests are small and have strong disease resistance; the crown and root system are large, the amount of leaves is large, and has a good soil and water conservation and improvement of soil effects. It plays a very important role in protecting and improving the ecological envi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 金晓玲何平胡希军张日清廖飞勇汪灵丹
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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