Agent for preventing reduction in production of collagen, and external composition for skin and cosmetic each comprising same
A technology for external composition and inhibitor, applied in the field of skin external composition and cosmetics, to achieve the effects of QOL improvement, prevention or improvement of wrinkles, and elasticity improvement
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[0068] The present invention will be specifically described based on the following examples, but the present invention is not limited to these examples.
[0069] (1) Preparation of bird's nest samples
[0070] (1-1) Bird's nest enzymatic hydrolyzate 1
[0071] Commercially available unbleached bird's nest is pulverized with a pulverizer to obtain bird's nest powder with a 100-mesh pass (below particle size 150 μm), add about 50 times the amount (mass) of water to the bird's nest powder, and swell at 5°C for 20 hours, and then sterilized at 121°C for 15 minutes.
[0072] After cooling the obtained treatment solution, the pH was adjusted, and an enzyme containing protease (trade name "Pancleachin F", manufactured by Amano Pharmaceutical Co., Ltd.) was added in an amount of 2% by mass relative to the bird's nest powder, and reacted at 45° C. for 3 hours.
[0073] The enzyme reaction solution was adjusted to pH 7.0, then heated at 90° C. for 5 minutes to inactivate the enzym...
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