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Determination method of total 2-(2-phenethyl) chromone compound content

A technology of chromone compounds and measurement methods, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of unreported measurement methods and difficulty in determination, and achieve the effects of high accuracy, good repeatability, and easy operation

Active Publication Date: 2012-12-12
厦门名香生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of 2-(2-phenylethyl)chromones in different agarwoods varies greatly, and the existing methods for the determination of 2-(2-phenylethyl)chromones in agarwood are all aimed at a certain or The content of several 2-(2-phenylethyl)chromones is determined, so these methods are difficult to determine or compare the total 2-(2-phenylethyl)chromone content of different agarwood or agarwood extracts
At present, there is no public report on the determination method of total 2-(2-phenylethyl)chromone content in Agarwood or Agarwood extract

Method used

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  • Determination method of total 2-(2-phenethyl) chromone compound content
  • Determination method of total 2-(2-phenethyl) chromone compound content
  • Determination method of total 2-(2-phenethyl) chromone compound content

Examples

Experimental program
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Embodiment 1

[0024] Example 1: Determination of the content of total 2-(2-phenylethyl)chromones in Agarwood

[0025] Take 3 parts (about 0.2g) of dried agarwood powder (about 0.2g) of the test sample, weigh them accurately, place them in a Soxhlet extractor, add 100ml of methanol, and extract under reflux for 6h, filter the extract and transfer it to a 100ml volumetric flask, add Dilute methanol to the mark, shake well, and use it as the solution to be tested, and the labels are respectively I, II, and III.

[0026] Accurately weigh an appropriate amount of 6,7-dimethoxy-2-(2-phenylethyl)chromone reference substance, dissolve in methanol and dilute to 1.5625mg / L, 3.125mg / L, 6.25mg / L, 9.375mg respectively / L, 12.5mg / L, 15.625mg / L reference substance solution, and adopt spectrophotometer to measure its absorbance value at 230nm place respectively, according to the concentration of reference substance solution and its absorbance value draw standard curve ( figure 1 ).

[0027] After the sol...

Embodiment 2

[0028] Example 2: Determination of the content of 2-(2-phenylethyl)chromones in the methanol extract of Agarwood

[0029] Take 3 parts of dry test sample agarwood powder (about 0.2g, the same batch as the test sample used in Example 1), weigh them accurately, place them in a Soxhlet extractor, add 100ml of methanol, and extract under reflux for 6h , the extract was concentrated to dryness. Accurately weigh the methanol extract of agarwood for testing, add an appropriate amount of methanol to fully dissolve, filter and transfer to a 100ml volumetric flask, add methanol to make up to the mark, shake well, and use it as the solution to be tested. The labels are IV, V, and VI. After the solution to be tested was diluted by a certain factor, its absorbance value at 230 nm was measured by a spectrophotometer, and the standard curve was drawn as in Example 1. Finally, the content of total 2-(2-phenylethyl)chromones in the methanol extract of Agarwood agarwood was calculated from the...

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Abstract

The invention relates to a determination method of a total 2-(2-phenethyl) chromone compound content, comprising the following steps of: extracting and filtering an agilawood sample to obtain a solution to be determined; by taking 6, 7-dimethoxy-2(2-phenethyl) chromone as a reference substance, dissolving the solution to be determined with a same solvent, preparing standard solutions with different concentrations, determining a light absorption value under 230nm, and establishing a standard curve; and determining the light absorption value under 230nm after the solution to be determined is diluted, the total 2-(2-phenethyl) chromone compound content in the solution to be determined is calculated by the standard curve and a dilution multiple, and finally calculating the total 2-(2-phenethyl) chromone compound content in the agilawood sample. According to the determination method disclosed by the invention, operation is convenient; and by utilizing the characteristics that a 2-(2-phenethyl) chromone compound has a 2-(2-phenethyl) chromone mother nucleus and determining the total 2-(2-phenethyl) chromone compound content in an agilawood substance by adopting an ultraviolet spectrophotometric method, pertinence is strong, stability is good, accuracy is high, and repeatability is good.

Description

technical field [0001] The invention belongs to the technical field of chemistry and medicine, and relates to a method for determining the content of total 2-(2-phenylethyl)chromones, in particular to a method for determining the content of total 2-(2-phenylethyl)chromones in agarwood or agarwood extract. ) Determination method of chromone content. Background technique [0002] 2-(2-Phenylethyl)chromones are a class of characteristic compounds produced during the formation of the precious medicinal material Agarwood. There have been research reports (Naef, et al, 2011; Chen, et al, 2011), and a total of 54 2-(2-phenylethyl)chromone compounds have been isolated and identified from Agarwood medicinal materials. Except Agarwood, so far only a few 2-(2-phenylethyl)chromones have been isolated from Imperata cylindrica, Bothriochloa ischaemum and Cucumis melo L. var. reticulatus. 2-(2-phenylethyl) chromones have the structural characteristics of the general formula (I), wherein ...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 刘洋洋魏建和杨云张争陈旭玉
Owner 厦门名香生物科技有限公司
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