Reagent and test strip for diagnosing bovine subclinical mastitis and preparation method thereof

A technology of recessive mastitis and test strips, which is applied in the field of diagnosis of dairy cow recessive mastitis, can solve the problems of high false negatives, different subjective standards, and uncertainty of subjective judgments, and achieve high diagnostic accuracy and convenient use , low cost effect

Inactive Publication Date: 2012-12-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of diagnosis of mastitis, the CMT method or improved CMT method is currently used in China. The basic principle of these methods is that flocculent precipitation occurs after the diagnostic fluid is mixed with milk, and the degree of mastitis is judged according to the time and amount of precipitation. These methods are: There is uncertainty in subjective judgment, and it is difficult to accurately define the judgment standard. Different people have different subjective standards, and the results are also different. The false negative is high; the other is that the tester needs to have certain professional knowledge, which also limits its clinical application.

Method used

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  • Reagent and test strip for diagnosing bovine subclinical mastitis and preparation method thereof
  • Reagent and test strip for diagnosing bovine subclinical mastitis and preparation method thereof
  • Reagent and test strip for diagnosing bovine subclinical mastitis and preparation method thereof

Examples

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Effect test

Embodiment 1

[0027] The preparation of embodiment 1 diagnostic reagent

[0028] Take 1g of catechol violet, add 100mL of water to dissolve it, and set aside; take 5g of neutral red, add 100mL of water to dissolve it, set aside; take 10g of Nile Blue A, add 100mL of glacial acetic acid to dissolve it, set aside; take α-naphthol 5g of benzyl alcohol, add 100mL of glacial acetic acid to dissolve it, set aside; take 50g of litmus powder, add 40mL of 95% ethanol, reflux and boil for 1 hour, let it stand, pour off the supernatant, and treat the precipitate in the same way 2 times, each time with 30mL of ethanol, wash the residue with 10mL of water, pour off the washing solution, add 50mL of water to boil, let it cool, filter to obtain litmus solution, and set aside;

[0029] Mix the above-mentioned five kinds of spare solutions evenly, adjust the pH to 7.2 with hydrochloric acid or sodium hydroxide, add water to make up to 1000mL, and let stand for 15 days to obtain the diagnostic reagent.

Embodiment 2

[0030] The preparation of embodiment 2 diagnostic reagents

[0031] Take 0.2g of catechol violet, add 100mL of water to dissolve it, and set aside; take 1g of neutral red, add 100mL of water to dissolve it, set aside; take 5g of Nile Blue A, add 100mL of glacial acetic acid to dissolve it, set aside; take α-naphthalene Add 100 mL of glacial acetic acid to dissolve 1 g of phenol-benzyl alcohol, and set aside; take 20 g of litmus powder, add 40 mL of ethanol with a concentration of 95%, reflux and boil for 1 hour, let it stand, pour off the supernatant, and then treat it twice with the same method. Use 30mL of ethanol each time, wash the residue with 10mL of water, pour off the washing solution, add 50mL of water to boil, let it cool, filter to obtain litmus solution, and set aside;

[0032] Mix the above-mentioned five kinds of spare solutions evenly, adjust the pH to 7.5 with hydrochloric acid or sodium hydroxide, add water to make up to 1000mL, and let stand for 15 days to ob...

Embodiment 3

[0033] The preparation of embodiment 3 diagnostic reagents

[0034] Take 2g of catechol violet, add 100mL of water to dissolve it, and set aside; take 10g of neutral red, add 100mL of water to dissolve it, set aside; take 20g of Nile Blue A, add 100mL of glacial acetic acid to dissolve it, set aside; take α-naphthol 10g of benzyl alcohol, add 100mL of glacial acetic acid to dissolve it, and set aside; take 80g of litmus powder, add 40mL of ethanol with a concentration of 95%, reflux and boil for 1 hour, let it stand, pour off the supernatant, and then treat it twice with the same method. Use 30mL of ethanol once, wash the residue with 10mL of water, pour off the washing liquid, add 50mL of water to boil, let it cool, filter to obtain litmus solution, and set aside;

[0035] Mix the above-mentioned five prepared liquids, adjust the pH to 7.4 with hydrochloric acid or sodium hydroxide, add water to make up to 1000 mL, and let stand for 15 days to obtain a diagnostic reagent.

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Abstract

The invention discloses a reagent and a test strip for diagnosing bovine subclinical mastitis and a preparation method thereof. The reagent comprises the following components: catechol violet, neutral red, litmus, Nile blue A and alpha-naphtholbenzein. The invention further discloses a diagnostic test strip prepared from the reagent for diagnosing bovine subclinical mastitis and a usage method thereof. The diagnostic test strip provided by the invention has advantages of low cost, convenient usage and high diagnostic accuracy. Clinical diagnostic test results show that the test paper provided by the invention has an accuracy rate higher than 95% on detecting bovine subclinical mastitis, and can be applied to clinical detection of bovine subclinical mastitis.

Description

technical field [0001] The present invention relates to a kind of diagnostic reagent, relate in particular to a kind of reagent for diagnosing dairy cow recessive mastitis and the test paper prepared by this reagent, the present invention further relates to their preparation method and in clinical diagnosis dairy cow recessive mastitis The application belongs to the field of diagnosis of milk cow recessive mastitis. Background technique [0002] Cow mastitis is one of the diseases with the highest incidence and the most serious economic loss in the dairy industry. The loss caused by mastitis alone in the world is as high as 35 billion U.S. dollars every year, and the loss caused by mastitis in China is about more than 5 billion U.S. dollars every year. According to whether there are visible changes in the udder and milk, cow mastitis is divided into two types: clinical mastitis and recessive mastitis. Recessive mastitis of dairy cows generally does not show clinical sympto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 高利肖建华范洪刚李艳飞张建涛魏成威刘本军王洪斌
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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