SiRNA inhibiting PPFIA1 gene expression, and its application

A technology of gene expression and sequence, applied in the field of siRNA
CN102827840BActive Publication Date: 2014-08-06广州安镝声生物医药科技有限公司
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州安镝声生物医药科技有限公司
Publication Date
2014-08-06

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an siRNA inhibiting the PPFIA1 gene expression, and its application. The positive-sense strand sequence of the siRNA is 5'-CCACAAAGCUCUGGAUGAA-3', the antisense strand sequence of the siRNA is 5'-UUCAUCCAGAGCUUUGUGG-3', and dTdT is dangled at the 3' segment of the siRNA seuqnce. The siRNA inhibiting the PPFIA1 gene expression can effectively silence the PPFIA1 gene and provides a technical scheme for researches on the functions of the PPFIA1 gene, and the silenced PPFIA1 gene can inhibit the cell growth and promote the cell apoptosis; and the siRNA can be used for preparing anti-leukemia medicines.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to an siRNA, in particular to an siRNA for inhibiting the expression of PPFIA1 gene and its application. Background technique

[0002] Chronic myelogenous leukemia (CML) is a malignant proliferative disease of hematopoietic stem cells mainly characterized by myelogenous hyperplasia, and its characteristic cytogenetic marker is Ph chromosome t(9;22)(q34;q11 ). P210BCR-ABL fusion protein is the product of Ph chromosome, has strong tyrosine kinase activity, and is closely related to the occurrence of CML. Its production can lead to the continuous phosphorylation of a series of signaling proteins downstream of it, thereby causing hematopoietic stem cells Pathophysiological changes such as malignant proliferation, anti-apoptosis, and adhesion function defects occur. Epidemiological surveys show that the incidence of CML among leukemia in my country is 1 / 10 5 , second only to Jili and Jilin, ranking third, showing a clear increasing ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More