Method for inducing accumulation of stilbenes in grapes

Abstract

The invention relates to a method for inducing the accumulation of stilbene substances resveratrol, resveratrol glycosides and pterostilbene in grape leaves, grape stems and grape fruits, including the preparation of hydrogen peroxide inducer, the preparation of grape in vitro and in vitro materials A method related to spraying treatment and immersion treatment of isolated grape materials. The technical scheme of the invention can effectively, specifically and comprehensively induce the content of stilbene substances in the above materials to increase, and has the characteristics of simple and safe operation, and is convenient for large-scale application in township enterprises with abundant grape resources. The invention can not only increase the quality of grapes but also increase the comprehensive utilization of grape planting waste, and can also make vines develop certain resistance to pathogenic bacteria and reduce losses caused by diseases.

Description

technical field [0001] The invention relates to a method for inducing the accumulation of stilbene substances resveratrol, resveratrol glycosides and pterostilbene in grape leaves, grape stems and grape fruits. Background technique [0002] Stilbenes are important constituents of phytoalexins, which exist in some medicinal plants and edible plants, such as cassia, hellebore, knotweed, grapes, and peanuts. Its biosynthesis depends on the secondary metabolic pathway of plant phenylpropane, and the produced stilbene substances, chalcones, lignin and other products are used as phytoalexins, UV protection factors, flower and fruit pigments, cell wall structural components and signal transduction molecules, etc. , play an important role in the physiological and pathological processes of plant stress [1] . Resveratrol is the most important substance synthesized by stilbene synthase (STS). Other key enzymes in this pathway include phenylalanine ammonia lyase (PAL), cinnamate-4-hy...

AI Technical Summary

Problems solved by technology

[0008] When analyzing the above-mentioned technical scheme of chemically inducing grape resveratrol accumulation, it was found that there were the following deficiencies: i. The effect is not good; ii. The above-mentioned induction scheme has a low specificity on the synthetic pathway of stilbenes, which may affect the quality and yield of grapes during the in vivo induction process; iii. The induction characteristics of glycosides and pterostilbene are unknown; iv. The above induction scheme does not take into account the difficulty of actual operation. In field operations, the selection of materials and the use of inducers determine the induction cost. Induction techniques such as stem injection, phosphonium aluminum in conjunction with UV light treatment, and copper sulfate in conjunction with chitosan are time-consuming and laborious to implement, and large-scale induction cannot be achieved; v. The above induction scheme does not take into account the residual toxicity of the inducer in soil and plants, The phytotoxicity of acidic aluminum and soluble copper salts in agricultural production has been recognized, and hormone reagents also have certain residual toxicity. These inducing reagents will accumulate in the soil during use, causing certain environmental toxicity, affecting subsequent crop planting, and also It will remain in plants and fruits, affecting the health of eaters; vi. The above induction scheme does not take into account the impact on operators when it is applied in the field. Acidic aluminum, phosphonium aluminum, copper sulfate, and plant hormones all have certain animal toxicity , the actual operation may cause certain poison to the operator, and even produce retention in the fruit, which is potentially toxic

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