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Lactobacillus rhamnosus probiotic and preparation method thereof

A technology of Lactobacillus rhamnosus and prebiotics, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of not being able to meet the needs of the market well, weak resistance of Lactobacillus, and inconvenient production, etc., to achieve high temperature Granulation, good effect, low cost effect

Inactive Publication Date: 2013-01-09
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN101507466A discloses a biological prebiotic and a preparation method thereof. The strain liquid is a quadruple bacteria mainly composed of a lactobacillus liquid accounting for 50-60% by weight, but the lactobacillus has weak resistance and a short shelf life , which brings some inconvenience to production, and cannot well meet the needs of the market

Method used

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Embodiment 1

[0028] Lactobacillus rhamnosus probiotic and its preparation method, the steps are as follows:

[0029] (1) Strain activation: Inoculate the Lactobacillus rhamnosus LT22 strain in the activation medium, and then stand for 36 hours in a 35±1°C incubator, which is the first-level bacterial liquid of Lactobacillus rhamnosus LT22; its activation The medium is composed of 1% peptone, 0.5% beef extract, 0.5% yeast extract, 2% glucose, 0.2% K 2 HPO 4 , 0.5% sodium acetate, 0.2% triammonium citrate, 0.06% MgSO 4 ·7H 2 O, 0.02% MnSO 4 ·H 2 O, 0.1% Tween 80 and the balance water composition;

[0030] (2) Strain expansion: the Lactobacillus rhamnosus LT22 first-level bacterial solution is connected to the expansion medium according to the inoculation amount of 5%, and it is allowed to stand in a 35±1℃ incubator for 60 hours and shake it every 12 hours. The culture medium, which is passed continuously for 2 generations, is Lactobacillus rhamnosus LT22 secondary and tertiary bacterial liquid; t...

Embodiment 2

[0039] Lactobacillus rhamnosus probiotic and its preparation method, the steps are as follows:

[0040] (1) Strain activation: Inoculate the Lactobacillus rhamnosus LT22 strain in the activation medium, and then stand for 24 hours in a 35±1℃ incubator, which is the first-level bacterial liquid of Lactobacillus rhamnosus LT22; its activation The medium is composed of 1% peptone, 0.5% beef extract, 0.5% yeast extract, 2% glucose, 0.2% K 2 HPO 4 , 0.5% sodium acetate, 0.2% triammonium citrate, 0.06% MgSO 4 ·7H 2 O, 0.02% MnSO 4 ·H 2 O, 0.1% Tween 80 and the balance water composition;

[0041] (2) Strain expansion: the Lactobacillus rhamnosus LT22 first-level bacterial solution is connected to the expansion medium according to the inoculation amount of 5%, and it is allowed to stand in a 35±1℃ incubator for 48 hours and shake it every 12 hours. The culture medium, which is passed continuously for 2 generations, is Lactobacillus rhamnosus LT22 secondary and tertiary bacterial fluid; ino...

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Abstract

The invention relates to a lactobacillus rhamnosus probiotic and a preparation method thereof and belongs to the technical field of microecologics and feed additives. The lactobacillus rhamnosus probiotic consists of a lactobacillus rhamnosus LT22 culture, a flocculating agent, a freeze-drying protective agent and a filling material. The preparation method of the lactobacillus rhamnosus probiotic comprises the steps of strain activation, strain culture expansion, bacterial cell flocculation, freeze drying of bacterial cells, filling material adsorption and mixing of two preparations. The lactobacillus rhamnosus probiotic prepared by means of the method contains bacterial cells with strong immunity, lactic acid, bacteriostatic active substance, soyabean protein, bran and other beneficial components, can promote digestion of protein, fat and carbohydrate in feeds, improve feed utilization ratio and promote animal growth, prevents pathogenic microorganisms from invading, inhibits growth of harmful bacteria, maintains the microecological balance of digestive tracts, stimulates organism immunity system response, improves the activity and the antibody level of immune cells, enables disease resistance of animals to be improved, and has very wide market prospect.

Description

technical field [0001] The invention relates to a microbial feed additive, in particular to a lactobacillus rhamnosus prebiotic and a preparation method thereof, and belongs to the technical field of microecological preparations and feed additives. Background technique [0002] At present, due to concerns about food safety, people have paid attention to the side effects of antibiotics as feed additives. The international community has proposed to reduce the use of antibiotic growth promoters or cancel the use of feed antibiotics. Probiotics are a kind of ideal substitutes of feed antibiotics developed under this premise. [0003] In the production of probiotics, the role of Lactobacillus in maintaining the health of humans and animals has attracted more and more attention. Lactobacillus is a common bacterium in the intestinal tract of animals. Lactobacillus probiotics have many functions such as nutrition, health care, improving body resistance, antagonizing pathogenic bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16
Inventor 刘颖徐春厚谢为天邝雪梅赵生才
Owner GUANGDONG OCEAN UNIVERSITY
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