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Stephanotis total alkaloid extract as well as preparation method and application thereof

A technology of total alkaloids and Baiyaozi, which is applied in the field of medicine, can solve the problems of large dose, unclear, and many ineffective impurities.

Active Publication Date: 2014-06-18
ANHUI JIUFANG DRUG RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the decoction of the decoction is cumbersome, backward, the dosage is large, and it cannot play a first-aid role, and the active ingredients are unstable, unclear, and there are too many invalid impurities, which is not conducive to the promotion and clinical application of Baiyaozi in the treatment of leukopenia. Therefore, it is imperative to develop high-standard, high-efficiency, long-acting, quick-acting, and safe drugs for the treatment of leukopenia.

Method used

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  • Stephanotis total alkaloid extract as well as preparation method and application thereof
  • Stephanotis total alkaloid extract as well as preparation method and application thereof
  • Stephanotis total alkaloid extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Extraction method of Baiyaozi total alkaloids:

[0031] Take the prescribed amount of Baiyaozi plus 6 times of ethanol for countercurrent dynamic heating extraction, each 3 hours, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa), each 1ml is equivalent to the original 5 g of medicinal materials, dissolved in hot water, acidified with hydrochloric acid, stirred, allowed to stand, filtered to obtain a filtrate, refrigerated for 48 hours, filtered, alkalized with alkali, stirred, allowed to stand, filtered to obtain a filtrate, and the obtained medicinal liquid Molecular retention filtration is carried out through a ceramic membrane filter. The pore size of the ceramic membrane filter is 0.1 μm to 0.3 μm, and the obtained filtrate is the fine filtrate; Elution with ethanol, the eluate was collected, concentrated under reduced pressure, and then spray-dried to obtain a dry powder extract. The dry...

Embodiment 2

[0034] Extraction method of Baiyaozi total alkaloids:

[0035] Take the prescribed amount of Baiyaozi and add 8 times of ethanol to carry out countercurrent dynamic heating extraction, 2 hours each time, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa), each 1ml is equivalent to the original 4 g of medicinal materials, dissolved in hot water, acidified with hydrochloric acid, stirred, allowed to stand, filtered to obtain a filtrate, refrigerated for 48 hours, filtered, alkalized with alkali, stirred, allowed to stand, filtered to obtain a filtrate, and the obtained medicinal liquid Molecular retention filtration is carried out through a ceramic membrane filter, the pore size of the ceramic membrane filter is 0.1 μm to 0.2 μm, and the obtained filtrate is the fine filtrate; Elution with ethanol, the eluate was collected, concentrated under reduced pressure, and then spray-dried to obtain a dry powder ...

Embodiment 3

[0037] Extraction method of Baiyaozi total alkaloids:

[0038] Take Baiyaozi of the prescription and add 5 times of ethanol to carry out countercurrent dynamic heating extraction, each time for 4 hours, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa), each 1ml is equivalent to the original 5 g of medicinal materials, dissolved in hot water, acidified with sulfuric acid, stirred, allowed to stand, filtered to obtain a filtrate, refrigerated for 48 hours, filtered, alkalized with alkali, stirred, allowed to stand, filtered to obtain a filtrate, and the obtained medicinal liquid Molecular retention filtration is carried out through a ceramic membrane filter, the pore size of the ceramic membrane filter is 0.2 μm to 0.3 μm, and the obtained filtrate is the fine filtrate; the fine filtrate is passed through the treated ionic resin column, washed with water to remove impurities, and then washed with ethano...

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Abstract

The invention discloses a stephanotis total alkaloid extract as well as a preparation method of the stephanotis total alkaloid extract. The preparation method of the stephanotis total alkaloid extract comprises the following steps of: grinding the dried stephanotis, heating and extracting, and decompressing and condensing the filtrate; diluting with heating water, and acidifying and filtrating to obtain a filtrate; alkalifying and filtrating to obtain the filtrate, and performing molecular weight cut off filtration on the obtained filtrate through a ceramic membrane filter; extracting with n-butyl alcohol, decompressing and condensing; spray drying the concentrated solution to obtain a dried powder extract; adding ethanol for ultrasonic extraction, mixing the extracting solution, absorbing the extracting solution by a sephadex column, eluting with 90% to 95% of ethanol, segmentally collecting, identifying through a high performance thin layer chromatography, mixing the parts containing alkaloids, freezing at a low temperature, and separating out an alkaloid crystal crude product; and adding ethanol for dissolving, standing and freezing to obtain a stephanotis total alkaloid pure product. A stephanotis total alkaloid medicinal preparation provided by the invention has the effect of adjuvant therapy on leucopenia, in particular to leucopenia of the tumor patients caused by radiotherapy and radiotherapy, and the leucopenia caused by other medicines.

Description

technical field [0001] The invention mainly relates to the technical field of medicine, in particular to a total alkaloid extract of Baiyaozi and a preparation method and application thereof. Background technique [0002] Baiyaozi is the tuberous root of the fangchiaceae plant. It grows in fertile and moist grasses, shady places on hillside roads or in shrubs, and also on limestone mountains; harvested in summer and autumn, sliced ​​and sun-dried. Rich in resources, distributed in central and southern my country. It has the functions of clearing away heat and detoxifying; dispelling wind and relieving pain; cooling blood and stopping bleeding. Ancient Chinese medicine is used for treatment throat Swelling and pain, carbuncle due to heat toxin, rheumatic arthralgia, abdominal pain diarrhea , Hematemesis, epistaxis, traumatic bleeding, etc. The taste is bitter, cool. [0003] This product is commonly used in modern Chinese medicine to treat various types of infections, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4748A61K36/59A61P7/00
Inventor 邵旭何万龙李晓亮牛海军
Owner ANHUI JIUFANG DRUG RES INST CO LTD
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