Chinese tongue squamous cancer cell line (CTSC-1) and establishing method thereof

A technology of CTSC-1 and squamous epithelium is applied in the field of Chinese tongue squamous cell cancer cell line CTSC-1 and its establishment, which can solve the problems of high cell resistance and other issues

Inactive Publication Date: 2013-02-06
SUN YAT SEN UNIV
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Problems solved by technology

Some patients received chemotherapy before surgery, s...

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  • Chinese tongue squamous cancer cell line (CTSC-1) and establishing method thereof
  • Chinese tongue squamous cancer cell line (CTSC-1) and establishing method thereof
  • Chinese tongue squamous cancer cell line (CTSC-1) and establishing method thereof

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Embodiment Construction

[0057] The establishment method of the Chinese tongue cancer cell line CTSC-1 of the present invention will be further described in conjunction with specific examples below.

[0058] 1. Establishment and Characterization of Tongue Squamous Cell Carcinoma Cell Line CTSC-1

[0059] Material Description:

[0060] 1 clinical specimen

[0061] The oral tissues used in this experiment were all surgically excised specimens from Guanghua Stomatological Hospital Affiliated to Sun Yat-sen University.

[0062] 2 cell lines

[0063] Tongue squamous cell carcinoma CAL27 cell line used in this experiment was purchased from ATCC

[0064] 3 main instruments

[0065]

[0066]

[0067] 4. Main reagents

[0068]

[0069]

[0070] 5 main solutions and formulations

[0071] 5.1 Cell Culture

[0072] 1) 10×PBS Buffer: NaCl 80g, KCl 2g, NaCl 2 HPO 4 11.55g, KH 2 PO 4 2g, add about 800ml of ultrapure water, fully stir to dissolve with a magnetic stirrer, adjust the pH value to ...

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Abstract

The invention provides an establishing method of a Chinese tongue squamous cancer cell line (CTSC-1), and provides a material base for researching the characteristics of tongue squamous cancer and therapeutics thereof. The establishing method comprises the steps of: carrying out primary culture on the tongue squamous cancer cells by adopting a tissue explants adherent method; subculturing when the cell density reaches about 80 percent; carrying out RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection on stably subcultured cells to indicate that the cells express tongue squamous cancer cell symbolic molecules CK 8, 18 and 19; and detecting adherent cells through a western blot test to express proteins of the tongue squamous cancer cell symbolic molecules CK 8 and 18, therefore, proving on a molecular level the successful establishment of the CTSC-1.

Description

technical field [0001] The present invention relates to the technical field of human tongue squamous cell carcinoma cells, in particular to a Chinese tongue squamous cell carcinoma cell line CTSC-1 and its establishment method. Background technique [0002] 1.1 Incidence and prognosis of tongue squamous cell carcinoma [0003] Tongue cancer is the most common oral squamous cell carcinoma, usually occurring in the anterior two-thirds of the tongue. Tongue cancer has a high incidence in Southeast Asia, with the highest incidence in India, with an annual incidence rate of 9.4 / 1 million. Studies in recent years have shown that the total incidence of tongue cancer worldwide is gradually increasing, and there is a trend of younger age. Due to the histological characteristics of the site where it occurs and the biological characteristics of tongue cancer, tongue cancer restricts tongue movement in patients, resulting in difficulties in swallowing, speaking, and eating. In additi...

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Application Information

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IPC IPC(8): C12N5/09C12N5/095C12Q1/02
Inventor 张雁贺姮张海霞汪华
Owner SUN YAT SEN UNIV
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