Triterpene compound and its preparation method and application

A triterpene compound and mixed solution technology, which is applied in the direction of steroids, organic chemistry, drug combination, etc., can solve the problems of poor control of ingredients and efficacy, anti-diabetic and unclear anti-viral active ingredients of guava leaves, etc., to achieve The effect of high production efficiency, low cost and simple operation

Active Publication Date: 2015-07-29
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention provides three kinds of triterpene compounds to solve the technology that guava leaves as a whole are not well controlled in composition and efficacy in the prior art, and the active ingredients of guava leaves' anti-diabetic and anti-viral effects are not clear. problems; and, methods of preparation and applications thereof

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  • Triterpene compound and its preparation method and application
  • Triterpene compound and its preparation method and application
  • Triterpene compound and its preparation method and application

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preparation example Construction

[0034] The embodiment of the present invention also provides a method for preparing the triterpene compound of the above structural formula I, comprising the following steps:

[0035] Step S01, preparing guava leaf extract:

[0036] extracting guava leaves with an extractant to obtain a guava leaf extract;

[0037] Step 02, prepare the triterpene compound of structural formula I:

[0038] The extract is subjected to high performance liquid chromatography separation, the mobile phase in the high performance liquid chromatography separation is a methanol solution with a volume ratio of methanol to water of 75:25 to 85:15, and the flow rate of the mobile phase is 1-20ml / min, Collect the eluate whose retention time is 52.6 minutes to 58.8 minutes to obtain the triterpene compound of structural formula I.

[0039] Specifically, in the preparation method of the triterpenoid compound of structural formula I in the embodiment of the present invention, the guava leaves are not limite...

Embodiment 1

[0143] The preparation method of the triterpene compound of the embodiment of the present invention with structural formula I comprises the following steps:

[0144] 1. Take 2.65kg of dried guava leaves and crush them, and extract them with 30L of 95% ethanol (volume ratio of ethanol:water=95:5). The extraction steps are as follows: cold soak at 25°C (room temperature) for 12 hours, filter, take the supernatant as the extraction solution Ⅰ, collect the residue, and count it as the residue Ⅰ; then extract the residue Ⅰ with 30L of 70% ethanol, the extraction conditions are the same as above, and the extracted Liquid II, residue II. Combine the extract I and the extract II to obtain the total extract.

[0145] The total extract was distilled under reduced pressure at a water temperature of 40°C to obtain 253.6g primary extract.

[0146] 2. Dissolve 253.6g primary extract in water, purify with D101 macroporous adsorption resin column (10×120cm), and carry out gradient elution w...

Embodiment 2

[0156] The preparation method of the triterpene compound of the embodiment of the present invention with structural formula II comprises the following steps:

[0157] 1. Take 2.65kg of dried guava leaves and crush them, and extract them with 30L of 95% ethanol (volume ratio of ethanol:water=95:5). The extraction steps are as follows: cold soak at 25°C (room temperature) for 12 hours, filter, take the supernatant as the extraction solution Ⅰ, collect the residue, and count it as the residue Ⅰ; then extract the residue Ⅰ with 30L of 70% ethanol, the extraction conditions are the same as above, and the extracted Liquid II, residue II. Combine the extract I and the extract II to obtain the total extract.

[0158] The total extract was distilled under reduced pressure at a water temperature of 40°C to obtain 253.6g primary extract.

[0159] 2. Dissolve 253.6g primary extract in water, purify with D101 macroporous adsorption resin column (10×120cm), and carry out gradient elution ...

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Abstract

The present invention belongs to the technical field of medicine, and provides triterpene compounds, preparation methods and applications thereof. According to the present invention, the triterpene compound represented by a structure formula I has cytotoxic activity; the triterpene compound represented by a structure formula II has an anti-diabetes effect; the triterpene compound represented by a structure formula III has weak cytotoxic activity; and the preparation methods for the three triterpene compounds have characteristics of simple operation, low cost and high production efficiency, and are suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a triterpene compound and a preparation method and application of the compound. Background technique [0002] According to the 2001 edition of the Chinese Medicine Dictionary (Volume 2), page 2411, guava leaves are the dry leaves and leafy tender stems of the plant guava (guajava L.) of the family Myrtaceae (Myrtaceae). Famous chicken arrow tea, also known as guava, trumpet fruit. There are about 150 species of Guava, which are distributed in tropical America, and 2 species have been introduced in my country. [0003] Guava leaves are sweet and astringent, flat in nature and non-toxic. Go into spleen, stomach, large intestine, liver channel. It has the effects of promoting body fluid to quench thirst, eliminating annoyance, astringent and antidiarrheal, anti-inflammatory and hemostasis, antipruritic and deworming. Chinese and foreign folk medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61P35/00A61P3/10
Inventor 李晓帆李玲曾小斌王乃利何祥久
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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