Determination method for content of saccharide components in mongolian milkvetch root extract

A technology of astragalus extract and determination method, which is applied in the field of content determination of active ingredients in traditional Chinese medicine extracts, and can solve problems such as poor stability and low sensitivity

Active Publication Date: 2013-02-13
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, these methods have poo

Method used

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  • Determination method for content of saccharide components in mongolian milkvetch root extract
  • Determination method for content of saccharide components in mongolian milkvetch root extract
  • Determination method for content of saccharide components in mongolian milkvetch root extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Astragalus extract carbohydrate composition determination method:

[0062] Determination according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 edition).

[0063] The chromatographic conditions and system suitability test used PrevailTM Carbohydrate ES chromatographic column, with acetonitrile: water = 75: 25 as mobile phase, flow rate 0.8ml / min, column temperature 30 °C; detection was performed with WATERS 2420 ELSD detector, and the parameter was set to gain 10. Air pressure 25psi, drift tube 60°C, Neb heater: 60%. The number of theoretical plates should not be less than 2000 based on the chromatographic peak of sucrose.

[0064] Preparation of reference substance solution Accurately weigh the appropriate amount of fructose reference substance and sucrose reference substance dried by phosphorus pentoxide, and add water to prepare a mixed control solution containing 0.5mg and 2.5mg of fructose and sucrose respectively per mill...

Embodiment 2

[0068] Astragalus extract carbohydrate composition determination method:

[0069] Determine according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 edition).

[0070] Chromatographic conditions and system suitability test using PrevailTM Carbohydrate ES chromatographic column, with acetonitrile: water = 75; 25 as mobile phase, flow rate 0.8ml / min, column temperature 30 ℃; detection with Alltech ELSD 2000 detector, gas flow rate: 2.5 ml / min, drift tube 105°C. The number of theoretical plates calculated based on the sucrose chromatographic peak should not be less than 4000.

[0071] Follow the same method as in Example 1.

Embodiment 3

[0073] Astragalus extract carbohydrate composition determination method:

[0074] Determine according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 edition).

[0075] The chromatographic conditions and system suitability test used PrevailTM Carbohydrate ES chromatographic column, with acetonitrile: water = 70: 30 as mobile phase, flow rate 0.8ml / min, column temperature 30 ℃; detection with Alltech ELSD 2000 detector, gas flow rate: 2.5 ml / min, drift tube 105°C. The number of theoretical plates calculated based on the sucrose chromatographic peak should not be less than 4000.

[0076] Follow the same method as in Example 1.

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Abstract

The present invention relates to a determination method for content of active ingredients in a traditional Chinese medicine extract, particularly to a determination method for content of saccharide components in a mongolian milkvetch root extract. The method comprises the following steps: 1) preparing a saccharide component control solution; 2) dissolving a mongolian milkvetch root extract, adding the dissolved mongolian milkvetch root extract to a small solid-phase extraction column, carrying out water elution, and collecting eluent; 3) adopting a high performance liquid chromatography method to carry out sample determination, wherein a PrevailTM Carbohydrate ES chromatographic column is adopted, and a certain proportion of an organic solvent and water are adopted as a mobile phase to carry out elution; 4) adopting an ELSD detector to carry out detection; and 5) adopting an external standard two-point method logarithmic equation to respectively calculate fructose content and sucrose content. The determination method has characteristics of simple operation, rapidness, strong specificity, high separation, good accuracy and good precision, and is suitable for quality control of the mongolian milkvetch root extract.

Description

Technical field: [0001] The invention relates to a method for measuring the content of active ingredients in a traditional Chinese medicine extract, in particular to a method for measuring the content of sugar components in an extract of Astragalus membranaceus, which is one of the intermediates of Qishen Yiqi dripping pills. Background technique: [0002] Astragalus membranaceus, a traditional Chinese medicinal material, is the root of Astragalus mongolica and Astragalus membranaceus, which have the functions of invigorating qi and strengthening the surface, diuresis and swelling, detoxifying and discharging pus, and promoting muscle growth. The medicinal use of Astragalus has a history of more than 2,000 years. Modern research shows that Astragalus contains saponins, sucrose, polysaccharides, various amino acids, folic acid, selenium, zinc, copper and other trace elements. It can enhance the immune function of the body, protect the liver, diuretic, anti-aging, anti-stress,...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 刘岩徐波牛涛陈红高阳
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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