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Determination method of carbohydrates in compound salvia miltiorrhiza extract

A technology of compound salvia miltiorrhiza and determination method, which is applied in the field of content determination of sugar components in compound salvia miltiorrhiza extract, and can solve the problems of poor stability and low sensitivity

Active Publication Date: 2015-11-25
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have poor stability and low sensitivity

Method used

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  • Determination method of carbohydrates in compound salvia miltiorrhiza extract
  • Determination method of carbohydrates in compound salvia miltiorrhiza extract
  • Determination method of carbohydrates in compound salvia miltiorrhiza extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Determination method of carbohydrate components in compound salvia miltiorrhiza extract:

[0069] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0070] The chromatographic conditions and system suitability test used PrevailTMCarbohydrateES chromatographic column, with acetonitrile as mobile phase A, water as mobile phase B, flow rate 0.8ml / min, column temperature 30°C; WATERS2420ELSD detector was used for detection, parameters were set to gain 10, air pressure 25psi, drift tube 60°C, Nebheater: 60%. The number of theoretical plates should be no less than 4000 based on the chromatographic peak of sucrose, and the chromatographic resolution of fructose and glucose should be no less than 2.0.

[0071] time (minutes)

Acetonitrile

water

0

75

25

20

75

25

23

60

40

30

60

40

33

75

25

38

75

25

[...

Embodiment 2

[0076] Recipe: Salvia 20%, Panax notoginseng 79%, Borneol 1%.

[0077] Step 1: decoct the salvia miltiorrhiza and notoginseng with 5 times the weight of alkaline water with a pH value of 8.3 for 2 hours, filter, add 4 times the amount of water to the dregs of the medicine and decoct for 1 hour, and mix the extract.

[0078] Step 2, the extract is concentrated and dried.

[0079] Determination method of carbohydrate components in compound salvia miltiorrhiza extract:

[0080] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0081] Chromatographic conditions and system suitability test using PrevailTMCarbohydrateES chromatographic column, with acetonitrile as mobile phase A, water as mobile phase B, flow rate 0.8ml / min, column temperature 30°C; detection with AlltechELSD2000 detector, gas flow rate: 2.5ml / min , drift tube 105°C. The number of theoretical plates calculated based on the sucrose chromatographic p...

Embodiment 3

[0086] Formula: Salvia 97%, Panax notoginseng 2%, Borneol 1.0%.

[0087] Step 1: decoct the salvia miltiorrhiza and notoginseng with 5 times the weight of alkaline water with a pH value of 8.3 for 2 hours, filter, add 4 times the amount of water to the dregs of the medicine and decoct for 1 hour, and mix the extract.

[0088] Step 2: Concentrate the extract, precipitate with alcohol, separate the supernatant, and filter.

[0089] Step 3, recovering ethanol and drying.

[0090] Determination method of carbohydrate components in compound salvia miltiorrhiza extract:

[0091] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0092] The chromatographic conditions and system suitability test used PrevailTMCarbohydrateES chromatographic column, with ethanol as mobile phase A, water as mobile phase B, flow rate 0.8ml / min, column temperature 30°C; WATERS2420ELSD detector was used for detection, parameters were set to ...

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Abstract

The invention relates to a method for determining the contents of carbohydrate components in a traditional Chinese medicinal extract, and especially relates to a method for determining the contents of carbohydrate components in a compound Salvia miltiorrhiza extract. The method comprises the following steps: 1, preparing a carbohydrate component reference substance solution; 2, dissolving the compound Salvia miltiorrhiza extract, adding the obtained Salvia miltiorrhiza extract solution into a small solid phase extraction column, eluting with water, and collecting the obtained eluate; 3, carrying out sample determination through adopting high performance liquid chromatography, and carrying out gradient elution through adopting a PrevailTM Carbohydrate ES chromatographic column and adopting a certain ratio of an organic solvent to water as a mobile phase; 4, detecting through using an ELSD detector; and 5, calculating according to an external standard two-point process logarithm equation to respectively obtain the fructose content, the glucose content and the sucrose content. The detection method has the advantages of simple and fast operation, strong specialization, high resolution, and good accuracy and precision, and is suitable for the control of the quality of the compound Salvia miltiorrhiza extract.

Description

Technical field: [0001] The invention relates to a method for determining the content of active ingredients in a traditional Chinese medicine extract, in particular to a method for determining the content of sugar components in a compound salvia miltiorrhiza extract, which is the raw material of the compound salvia miltiorrhiza dripping pill. Background technique: [0002] Compound Danshen preparations are cardiovascular drugs prepared from Danshen, Panax notoginseng and borneol. Currently, there are Compound Danshen Capsules, Compound Danshen Tablets and Compound Danshen Dripping Pills. In particular, compound Danshen dripping pills are widely used in the prevention, treatment and first aid of coronary heart disease and angina pectoris. Now it has become one of the leading brands in the domestic cardiovascular market. [0003] Compound Danshen extract is the extract of Danshen and Panax notoginseng. It is an intermediate of compound Danshen preparation and belongs to the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 刘岩徐波牛涛陈红高阳
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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