Preparation method and application of tsinling larkspur root extractive

The technology of an extract, Yunwuqi, is applied in the field of medicine to achieve the effect of simple preparation method and low price

Inactive Publication Date: 2013-03-13
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Indications for headache, low back pain, abdominal pain, and the anti-tumor effect of Yunwuqi extract has not been reported

Method used

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  • Preparation method and application of tsinling larkspur root extractive

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Embodiment Construction

[0013] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0014] (1) Weigh 500g of crushed Yunwuqi, add 2.5L of 95% ethanol to mix, and soak for 0.5h;

[0015] (2) At 60°C, use a Soxhlet extractor to reflux extraction for 3 hours, repeating 3 times;

[0016] (3) Concentrate the extract to nearly dryness with a rotary evaporator to obtain 5.0 g of extract, which is dissolved in DMSO.

[0017] Collect the in vitro cultured liver cancer (HepG-2), gastric cancer (MGC-803) and lung cancer (A-549) cells in the logarithmic growth phase, and adjust the density to 1×10 5 cells / mL, inoculated in 96-well plate at 200 μL / well, set at 37°C, 5% CO 2 After culturing in an incubator saturated with water vapor for 24 hours, the extract of Yunwuqi was added to the culture medium at the final concentration of 0.05, 0.5 and 5 μg / mL, and the activity of tumor cells was detected by MTT method. The results showed ...

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Abstract

The invention discloses a preparation method and application of a tsinling larkspur root extractive. The preparation method comprises the following steps of: (1) weighing crushed tsinling larkspur root, adding 95% of ethanol, and dipping for 0.5 hour; (2) carrying out reflux extraction for 3 hours at 60 DEG C in three times by a soxhlet extractor, and repeating the step three times; and (3) concentrating the extracting solution obtained in step (2) by a rotary evaporator until the extracting solution is nearly dry, so as to obtain the extractive, and then dissolving by DMSO (Dimethylsulfoxide). The preparation method of the tsinling larkspur root extractive is simple; and the tsinling larkspur root extractive is low in price and has a certain antineoplastic effect, so that the tsinling larkspur root extractive has a great application value for preparation of antineoplastic drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method and application of Yunwuqi extract. Background technique [0002] Tumor is one of the main diseases threatening human health at present, and chemotherapy is a main method of tumor treatment at present. Traditional synthetic chemical anticancer drugs are expensive and have severe side effects, which restrict their application. Traditional Chinese herbal medicine has the characteristics of multi-target, strong specificity and less toxic side effects. The research and development of anticancer drugs of Chinese herbal medicine and its active ingredients has become a research hotspot at home and abroad because of its huge application value and market prospect. [0003] Delphinium giraldii is a perennial herb distributed in Shaanxi, Henan, Gansu, Sichuan, Shanxi, Hubei, Ningxia and other places. It grows in areas with an altitude of 960 meters to 2000 meters, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P35/00
Inventor 罗明志罗雯李莺
Owner XIAN UNVERSITY OF ARTS & SCI
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